Laser & Optoelectronics Progress, Volume. 55, Issue 3, 030006(2018)
Analysis of New Super-Resolution Microscopy Technology
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![Mammalian brain circuitry observed by proExM[14]. (a) Pre-expansion and (b) post-expansion wide field imaging pictures when virus with green fluorescent protein injected in cortex of macaque; (c) image taken with a confocal microscope and stereoscopically rendered of the boxed region in Fig. 2(b)](/Images/icon/loading.gif){kind=icon}
Fig. 2. Mammalian brain circuitry observed by proExM[14]. (a) Pre-expansion and (b) post-expansion wide field imaging pictures when virus with green fluorescent protein injected in cortex of macaque; (c) image taken with a confocal microscope and stereoscopically rendered of the boxed region in Fig. 2(b)
Fig. 5. Fluorescent particles with diameter of 100 nm obtained by PSIM[18]. (a) Conventional fluorescence microscopic image; (b) reconstructed PSIM image; (c) corresponding scanning electron microscope image; (d) Fourier transform of Fig. 5(a); (e) Fourier transform of Fig. 5(b); (f) fluorescence intensity distribution
Table 1. Main differences between chip-based ESI and dSTORM technologies
View tableTable 1. Main differences between chip-based ESI and dSTORM technologies
Parameter Chip-based ESI technology Chip-based dSTORM technology Acquisition time <25 s 8-30 min Resolution with ×20 lens /nm 340 <140 Resolution with ×60 lens /nm 110 <50
Table 2. Main differences between SPoD and SDOM technologies based on fluorescence polarization microscope
View tableTable 2. Main differences between SPoD and SDOM technologies based on fluorescence polarization microscope
Technology Algorithm Orientation information Acquisition time Resolution SPoD SPEED No About 300 ms About 100 nm SDOM Polarization-variant deconvolution Yes About 200 ms About 100 nm
Table 3. Comparison of several new imaging methods
View tableTable 3. Comparison of several new imaging methods
Method Name Spatial resolution Time resolution Specimen requirement Expansion microscopy ExM 70 nm Depend on what kind of microcopy used Escherichia coli, mammalian cells, mouse cortex and brain hippocampus, etc iExM 25 nm Surface enhanced microscopy PSIM 2.6-flod of epi-fluorescence Depend on what kind of microcopy used Just proved in beads CWN 50 nm (with dSTORM) 8 frame·s-1 (with ESI) Stationary samples that need specific preparation Fluorescence polarization microscopy SPoD 100 nm 3 frame·s-1 Limited by densely labeled and heterogeneously samples SDOM 100 nm 5 frame·s-1 Limited by densely labeled, and homogenously orientated samples polar-dSTORM 10 nm 2-40 min Stationary samples that need specific preparation
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Lujia Jin, Yang He, Luxi Qu, Chi Zhang, Meiqi Li, Peng Xi. Analysis of New Super-Resolution Microscopy Technology[J]. Laser & Optoelectronics Progress, 2018, 55(3): 030006
Paper Information
Category: General
Received: Aug. 25, 2017
Accepted: --
Published Online: Sep. 10, 2018
The Author Email: Li Meiqi ( limeiqi@pku.edu.cn)
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