Estimation of Chlorophyll Content in Maize Leaves Based on Optimized Area Spectral Index

Yu-zhe TANG; Mei HONG; Jia-yong HAO; Xu WANG; He-jing ZHANG; Wei-jian ZHANG; Fei LI

doi:10.3964/j.issn.1000-0593(2022)03-0924-09

Spectroscopy and Spectral Analysis, Volume. 42, Issue 3, 924(2022)

Estimation of Chlorophyll Content in Maize Leaves Based on Optimized Area Spectral Index

Yu-zhe TANG*... Mei HONG, Jia-yong HAO, Xu WANG, He-jing ZHANG, Wei-jian ZHANG and Fei LI*; |Show fewer author(s)

College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, Inner Mongolia Agricultural University, Huhhot 010018, China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(10)

Fig. 1. Descriptive statistics of leaf SPAD values in different growth stages of maize

Download full size

View in Article

Fig. 2. Contour maps of the coefficient of determination (R²) between leaf chlorophyll content and OCAI index
(a): V6 stage; (b): V12 stage; (c): VT stage; (d): R3 stage

Download full size

View in Article

Fig. 3. Contour maps of the coefficient of determination (R²) between leaf chlorophyll content and OTVI index
(a): V6 stage; (b): V12 stage; (c): VT stage; (d): R3 stage

Download full size

View in Article

Fig. 4. Contour maps of the coefficient of determination (R²) between leaf chlorophyll content and ONDDA index

Download full size

View in Article

Fig. 5. The linear relationship between leaf chlorophyll contents in four growth stages and the top 6 spectral indices of estimation ability

Download full size

View in Article

Fig. 6. Comparison between observed and predicted leaf chlorophyll contents based on six spectral indices

Download full size

View in Article

Fig. 7. The sensitive bands of OCAI, OTVI and ONDDA at different growth stages

Download full size

View in Article

Table 1. Spectral indices

View table

View in Article

Table 1. Spectral indices

光谱指数	算法	参考文献
三角形面积指数
Triangular greenness index (TGI)	-0.5[(670-480)(R₆₇₀-R₅₅₀)-(670-550)(R₆₇₀-R₄₈₀)]	Hunt et al. (2013)
Triangular chlorophyll index (TCI)	1.2(R₇₀₀-R₅₅₀)-1.5(R₆₇₀-R₅₅₀)(R₇₀₀/R₆₇₀)⁰^.⁵	Haboudane et al. (2008)
Triangle vegetation index (TVI)	0.5×[120×(R₇₅₀-R₅₅₀)-200×(R₆₇₀-R₅₅₀)]	Broge and Leblanc (2000)
Modified triangular vegetation index 1 (MTVI1)	1.2×[1.2×(R₈₀₀-R₅₅₀)-200×(R₆₇₀-R₅₅₀)]	Haboudane et al.(2004)
Modified triangular vegetation index 2 (MTVI2)	$\frac{1.5 \times [1.2 \times (R_{800} - R_{500}) - 2.5 \times (R_{670} - R_{550})]}{\sqrt[]{(2 \times R_{800} {+ 1)}^{2} - (6 \times R_{800} - 5 \times \sqrt[]{R_{670}}) - 0.5}}$	Haboudane et al. (2004)
Optimized triangle vegetation index(OTVI)	0.5×[(λ₂-550)×(Rλ₁-Rλ₂)-(Rλ₂-R₅₅₀)×(λ₁-λ₂)]^[1]	Li et al. (2013)
叶绿素吸收面积指数
Chlorophyll absorption ratio index (CARI)	[R₇₀₀×abs(a×670+R₆₇₀+b)]/R₆₇₀×(a²+1 ${)^{0.5}}^{[2]}$	Kim et al. (1994)
Modified chlorophyll absorption reflectance index (MCARI)	[(R₇₀₀-R₆₇₀)-0.2(R₇₀₀-R₅₅₀)](R₇₀₀/R₆₇₀)	Daughtry et al. (2000)
Modified chlorophyll absorption reflectance index 1 (MCARI1)	1.2×[2.5×(R₈₀₀-R₆₇₀)-1.3×(R₈₀₀-R₅₅₀)]	Haboudane et al. (2004)
Modified chlorophyll absorption reflectance index 2 (MCARI2)	$\frac{1.2 \times [2.5 \times (R_{800} - R_{670}) - 1.3 \times (R_{800} - R_{550})]}{\sqrt[]{(2 \times R_{800} {+ 1)}^{2} - (6 \times R_{800} - 5 \times \sqrt[]{R_{670}}) - 0.5}}$	Haboudane et al. (2004)
MCARI (705, 750)	[(R₇₅₀-R₇₀₅)-0.2(R₇₅₀-R₅₅₀)](R₇₅₀/R₇₀₅)	Wu et al. (2008)
Transformed chlorophyll absorption reflectance index (TCARI)	3×[(R₇₀₀-R₆₇₀)-0.2(R₇₀₀-R₅₅₀)(R₇₀₀/R₆₇₀)]	Haboudane et al. (2002)
TCARI (750, 705)	3×[(R₇₅₀-R₇₀₅)-0.2(R₇₅₀-R₅₅₀)(R₇₅₀/R₇₀₅)]	Wu et al. (2008)
Red-edge absorption chlorophyll area(RECA)	$\int_{680}^{780} \frac{R}{R_{780}}$	Ren et al. (2011)
Red-edge absorption valley area (REA)	$1 / 2 \times (R_{680 + Δ λ} - R_{680}) \times Δ λ^{[3]}$	Guo et al. (2017)
Optimized red-edge absorption valley area (OREA)	15×(3×R₇₆₀ -R₅₅₀)-20×(R₆₈₀+2R₇₂₀)	Wen et al. (2020)
Normalized area over reflectance curve (NAOC)	$1 - {\frac{\int_{a}^{b} ρ d λ}{ρ_{\max} (b - a)}}^{[4]}$	Delegido et al. (2010)
Chlorophyll absorption integral (CAI)	$\int_{600}^{735} {\frac{R_{si}}{R_{ei}}}^{[5]}$	N. Oppelt et al. (2007)
Optimized Chlorophyll absorption integral (OCAI)	$\int_{E}^{F} {\frac{R_{si}}{R_{ei}}}^{[6]}$	This study
波形变换指数
Area under curve Normamised to Maximal Band(ANMB)	AUC_500-800/MBD_500-800	Malenovsky et al. (2006)
Double-peak canopy nitrogen index (DCNI)	(R₇₂₀-R₇₀₀)/(R₇₀₀-R₆₇₀)/(R₇₂₀-R₆₇₀+0.03)	Chen et al. (2010)
Optimized double-peak canopy nitrogen index (ODCNI)	(R₇₂₀-R₇₀₀)/(R₇₀₀-R₆₇₀)/(R₇₂₀-R₆₇₀+n); n=[-1∶1]	This study
Difference index of the double-peak areas(DIDA)	(R₇₅₅+R₆₈₀-3×R₇₁₈)/(R₇₅₅-R₆₈₀)	Feng et al. (2014)
Ratio index of the double-peak areas(RIDA)	$\frac{RSDR}{LSDR}$	Feng et al. (2014)
Double-peak areas based on REP division(NDDA)	(R₇₅₅+R₆₈₀-2×R_REPig)/(R₇₅₅-R₆₈₀)	Feng et al. (2014)
Optimized double-peak areas based on REP division(ONDDA)	$(msubsup - msubsup) / msubsup$	This study

Table 2. Coefficient of determination (R²) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages

View table

View in Article

Table 2. Coefficient of determination (R²) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages

	EXP.1					EXP.2				EXP.3
	V6	V12	VT	R3	All	V12	VT	R3	All	VT	All
基于三角形面积的光谱指数
TGI	0.83	0.90	0.95	0.95	0.92	0.95	0.93	0.95	0.94	0.95	0.94
TCI	0.81	0.87	0.91	0.92	0.88	0.95	0.90	0.92	0.91	0.89	0.89
TVI	0.35	0.62	0.86	0.74	0.59	0.59	0.56	0.74	0.61	0.83	0.60
MTVI1	0.37	0.65	0.85	0.75	0.62	0.61	0.55	0.78	0.63	0.84	0.63
MTVI2	0.65	0.91	0.94	0.94	0.85	0.88	0.92	0.93	0.91	0.93	0.87
OTVI	0.85	0.94	0.98	0.95	0.95	0.90	0.95	0.97	0.94	0.95	0.95
基于叶绿素吸收面积的光谱指数
CARI	0.70	0.35	0.90	0.93	0.65	0.92	0.92	0.93	0.91	0.89	0.87
MCARI	0.80	0.87	0.87	0.89	0.85	0.94	0.88	0.91	0.90	0.86	0.87
MCARI1	0.57	0.66	0.85	0.75	0.62	0.61	0.55	0.79	0.63	0.84	0.63
MCARI2	0.65	0.91	0.94	0.95	0.85	0.88	0.92	0.93	0.91	0.93	0.87
MCARI(705, 750)	0.67	0.83	0.96	0.93	0.86	0.83	0.90	0.95	0.88	0.95	0.87
TCARI	0.80	0.87	0.94	0.94	0.90	0.95	0.92	0.94	0.93	0.92	0.92
TCARI(750, 705)	0.37	0.56	0.89	0.71	0.67	0.81	0.85	0.71	0.79	0.86	0.73
RECA	0.58	0.73	0.93	0.93	0.81	0.85	0.88	0.94	0.88	0.93	0.84
CAI	0.38	0.56	0.82	0.88	0.63	0.93	0.89	0.89	0.88	0.88	0.67
REA	0.76	0.88	0.95	0.92	0.89	0.86	0.92	0.90	0.89	0.93	0.89
NAOC	0.81	0.90	0.97	0.95	0.93	0.92	0.95	0.96	0.94	0.94	0.91
OREA	0.81	0.89	0.97	0.95	0.92	0.89	0.83	0.96	0.92	0.95	0.92
OCAI	0.82	0.91	0.96	0.95	0.92	0.94	0.95	0.96	0.94	0.95	0.93
基于波形变换的光谱指数
ANMB	0.69	0.80	0.94	0.95	0.86	0.69	0.88	0.94	0.94	0.93	0.89
DCNI	0.64	0.75	0.93	0.91	0.83	0.86	0.93	0.80	0.85	0.92	0.84
DIDA	0.79	0.88	0.97	0.96	0.92	0.92	0.95	0.95	0.94	0.96	0.85
RIDA	0.80	0.87	0.96	0.93	0.89	0.87	0.93	0.88	0.89	0.97	0.93
NDDA	0.82	0.89	0.95	0.95	0.92	0.95	0.91	0.92	0.91	0.92	0.88
ODCNI	0.65	0.77	0.94	0.92	0.84	0.87	0.94	0.81	0.86	0.93	0.85
ONNDA	0.88	0.94	0.99	0.98	0.96	0.97	0.97	0.97	0.96	0.98	0.96

Table 3. Characteristics of the regression between observed and estimated SPAD values
View table
View in Article
Table 3. Characteristics of the regression between observed and estimated SPAD values
Spectral index R² RMSE NRMSE/% Slope
TGI 0.928 2.543 4.585 0.917
TCARI 0.912 2.794 5.077 0.907
ANMB 0.845 3.951 7.099 0.951
OTVI 0.936 2.403 4.116 0.973
OCAI 0.939 2.359 4.012 0.922
ONDDA 0.943 2.285 3.938 0.996

Tools

Get Citation

Copy Citation Text

Yu-zhe TANG, Mei HONG, Jia-yong HAO, Xu WANG, He-jing ZHANG, Wei-jian ZHANG, Fei LI. Estimation of Chlorophyll Content in Maize Leaves Based on Optimized Area Spectral Index[J]. Spectroscopy and Spectral Analysis, 2022, 42(3): 924

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites

Paper Information

Category: Orginal Article

Received: Feb. 28, 2021

Accepted: May. 14, 2021

Published Online: Apr. 19, 2022

The Author Email: TANG Yu-zhe (tangyuzhe9702@163.com)

DOI:10.3964/j.issn.1000-0593(2022)03-0924-09

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Spectral indices

Table 1. Spectral indices

Table 2. Coefficient of determination (R2) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages

Table 2. Coefficient of determination (R2) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages

Table 3. Characteristics of the regression between observed and estimated SPAD values

Table 3. Characteristics of the regression between observed and estimated SPAD values

微信扫一扫：分享

Table 2. Coefficient of determination (R²) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages

Table 2. Coefficient of determination (R²) of the linear relationships between leaf chlorophyll content and spectral indices in different growth stages