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Laser & Optoelectronics Progress, Volume. 55, Issue 3, 033004(2018)

Photoluminescence Properties of Single-Stranded Nucleic Acids with Different Base Sequences

Changneng Liang1, Jie Zhang1, Wen Xu1,2、*, Lan Ding1, Wei Qian1, and Xuan Li1
Author Affiliations
  • 1 School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, China
  • 1 School of Materials Science and Engineering, Yunnan University, Kunming, Yunnan 650091, China
  • 1 School of Physics and Astronomy, Yunnan University, Kunming, Yunnan 650091, China
  • 2 Institute of Solid State Physics, Chinese Academy of Sciences, Hefei, Anhui 230031, China
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    AbstractGet PDF(in Chinese)
    Figures&Tables (7)
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    Figures & Tables(7)
    Schematic of optical path of fluorescence detection

    Fig. 1. Schematic of optical path of fluorescence detection

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    Fluorescence spectra of two nucleic acid sample solutions and double distilled water

    Fig. 2. Fluorescence spectra of two nucleic acid sample solutions and double distilled water

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    Fluorescence spectra of two nucleic acid. (a) Sample 61191; (b) sample 61192

    Fig. 3. Fluorescence spectra of two nucleic acid. (a) Sample 61191; (b) sample 61192

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    (a) Relationship between fluorescence peak position and the excitation wavelength; (b) relationship between fluorescence peak intensity and the excitation wavelength

    Fig. 4. (a) Relationship between fluorescence peak position and the excitation wavelength; (b) relationship between fluorescence peak intensity and the excitation wavelength

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    Schematic of photoluminescence process

    Fig. 5. Schematic of photoluminescence process

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    Excitation spectra of highest intensity fluorescence peak of two nucleic acid samples

    Fig. 6. Excitation spectra of highest intensity fluorescence peak of two nucleic acid samples

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    • Table 1. Properties of nucleic acid samples

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      Table 1. Properties of nucleic acid samples

      SampleSequence5'-3'Number of basesequenceEmbellishPurificationwayTemperatureof melting /℃Concentration /(μmol·L-1)
      61191TAG-AAT-TCA-TGA-CAA-CAG-TTC-CTA-CGG-AT29NonePAGE58.25
      61192TAT-CGG-CCG-CTT-CCG-CCA-CCC-TGG-AAG-TAC-AGG-TTT-TCA-GCC-ACA-GCA-TTA-TCC-CT56NonePAGE73.75
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    Changneng Liang, Jie Zhang, Wen Xu, Lan Ding, Wei Qian, Xuan Li. Photoluminescence Properties of Single-Stranded Nucleic Acids with Different Base Sequences[J]. Laser & Optoelectronics Progress, 2018, 55(3): 033004

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    Paper Information

    Category: Spectroscopy

    Received: Jul. 27, 2017

    Accepted: --

    Published Online: Sep. 10, 2018

    The Author Email: Xu Wen (wenxu_issp@aliyun.com)

    DOI:10.3788/LOP55.033004

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology