To improve the precise of all automatic printing equipment, a simple and effective calibration method was proposed to calibrate the screen printing equipment based on a novel three-degree-of-freedom (3-DOF) planar parallel stage. Firstly, a vision-based measuring system was analyzed and calibrated, and its measurement accuracy was also evaluated by a laser interferometer. Then, the geometric errors of the 3-DOF planar parallel stage was analyzed and the frame of the parallel stage's movable platform, screw lead errors and some geometric errors were identified in steps based on a vision-based measuring system in the equipment. Furthermore, the orientation interpolation and alignment methods using a triangular area to position the alignment point were presented to satisfy the need for different product processes in the whole workspace. For the processing mentioned above, this calibration method can calibrate the equipment itself automatically without complicated identification for all geometric errors and the need for any background in parallel robot calibration. Experimental results after calibration show that the maximum position and orientation errors inside the workspace have been reduced from 161.6 μm and 2.232″ to 12.3 μm and 0.720″, respectively, which satisfies the equipment requirement for high accuracy.