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Journal of Inorganic Materials, Volume. 35, Issue 2, 187(2020)

Construction of Polyethyleneimine-modified Dual-mesoporous Silica Gene Carrier

Mei-Rong FANG1... Li-Mei QIN2, Xiao-Bo JIA2, Yong-Sheng LI2, De-Chao NIU2,* and Ze-Lan HU1,* |Show fewer author(s)
Author Affiliations
  • 1Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • 2School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China
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    TEM images of EDMSNs-PEI (a) and EDMSNs- NH2 (b)
    1. TEM images of EDMSNs-PEI (a) and EDMSNs- NH2 (b)

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    Zeta potentials (a) and particle size distributions (b) of EDMSNs, EDMSNs-PEI and EDMSNs-NH2
    2. Zeta potentials (a) and particle size distributions (b) of EDMSNs, EDMSNs-PEI and EDMSNs-NH2

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    N2 adsorption-desorption isotherms and the corresponding DFT pore size distributions of EDMSNs-PEI (a-b) and EDMSNs-NH2 (c-d)
    3. N2 adsorption-desorption isotherms and the corresponding DFT pore size distributions of EDMSNs-PEI (a-b) and EDMSNs-NH2 (c-d)

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    Electrophoresis diagram of EDMSNs-PEI/pDNA and EDMSNs-NH2/pDNA nanoparticles at different weight ratios (a), DNaseI digestion electrophoresis diagram (b) and zeta potentials (c) of EDMSNs-PEI/pDNA with M indicating DNA marker and C indicating naked pDNA
    4. Electrophoresis diagram of EDMSNs-PEI/pDNA and EDMSNs-NH2/pDNA nanoparticles at different weight ratios (a), DNaseI digestion electrophoresis diagram (b) and zeta potentials (c) of EDMSNs-PEI/pDNA with M indicating DNA marker and C indicating naked pDNA

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    Cytotoxicity assay of PEI 25kD, LIP2K and EDMSNs-PEI in 293T (a) and u87(b). * p<0.01, EDMSNs-PEI
    5. Cytotoxicity assay of PEI 25kD, LIP2K and EDMSNs-PEI in 293T (a) and u87(b). * p<0.01, EDMSNs-PEI

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    Transfection efficacy of EDMSNs-PEI/pDNA nanoparticle in 293T cell lines.
    6. Transfection efficacy of EDMSNs-PEI/pDNA nanoparticle in 293T cell lines.

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    Mei-Rong FANG, Li-Mei QIN, Xiao-Bo JIA, Yong-Sheng LI, De-Chao NIU, Ze-Lan HU. Construction of Polyethyleneimine-modified Dual-mesoporous Silica Gene Carrier[J]. Journal of Inorganic Materials, 2020, 35(2): 187

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    Paper Information

    Category: RESEARCH PAPER

    Received: Mar. 5, 2019

    Accepted: --

    Published Online: Jan. 27, 2021

    The Author Email: NIU De-Chao (dcniu@ecust.edu.cn), HU Ze-Lan (huzelan@ecust.edu.cn)

    DOI:10.15541/jim20190099

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology