Spectroscopy and Spectral Analysis, Volume. 40, Issue 11, 3579(2020)
Changes of Tumor Cell Components Induced by Pain-Related Neuropeptides SP Investigated by Synchrotron Radiation Infrared Microscopic Imaging Technology
Figures & Tables(6)
Table 1. The bands frequencies assigned for the IR spectra
View tableView in ArticleTable 1. The bands frequencies assigned for the IR spectra
Range
/cm-1Peak
position/cm-1Possible resolve 990~1 040 1 029 C—O—, C—O—H 1 040~1 070 1 054 C—O stretching 1 070~1 090 1 083 P=O stretching 1 150~1 190 1 165 C—O stretching 1 230~1 250 1 240 P=O stretching 1 430~1 470 1 455 —CH2 deformation 1 500~1 600 1 551 Amide Ⅱ 1 600~1 700 1 655 Amide Ⅰ 1 700~1 800 1 747 C=O stretching 2 910~2 950 2 930 C—H stretching 2 950~2 990 2 970 3 300~3 500 3 383 O—H, N—H stretching
Table 2. Spectral peak analysis of breast cancer cell lines
View tableView in ArticleTable 2. Spectral peak analysis of breast cancer cell lines
MDA-MB-231(Mean±SD) P-value MCF-7(Mean±SD) P-value Control(n=12) SP(n=12) Control(n=12) SP(n=12) Range
/cm-11 070~1 090 1 076.69±6.56 1 077.241±4.572 0.854 1 079.764±2.19 1 078.05±2.025 0.082 1 230~1 250 1 235.383±2.916 1 234.832±6.171 0.848 1 237.656±4.917 1 237.656±7.766 0.51 1 380~1 420 1 396.005±2.062 1 397.934±4.648 0.134 1 396.763±3.38 1 395.316±4.25 0.272 1 430~1 470 1 452.760±4.944 1 453.587±5.068 0.732 1 459.441±7.968 1 460.405±4.696 0.401 1 500~1 600 1 548.086±0.941 1 538.719±1.712 <0.001 1 547.432±3.082 1 538.03±4.25 0.002 1 600~1 700 1 651.678±4.705 1 639.555±3.419 0.001 1 647.235±4.426 1 636.869±3.391 <0.001 2 910~2 950 2 925.311±4.121 2 925.247±4.181 0.9 2 925.632±1.897 2 924.347±0.996 0.209 2 950~2 990 2 959.244±1.517 2 959.244±2.187 0.999 2 958.418±1.604 2 958.9±0.893 0.241 3 300~3 500 3 295.366±1.834 3 295.488±2.396 0.929 3 292.542±1.996 3 292.059±5.646 0.417
Table 3. Statistical analysis of spectral peak of breast cancer cell lines
View tableView in ArticleTable 3. Statistical analysis of spectral peak of breast cancer cell lines
MDA-MB-231(Mean±SD) P-value MCF-7(Mean±SD) P-value Control(n=12) SP(n=12) Control(n=12) SP(n=12) Range
/cm-11 070~1 090 0.52±0.129 0.581±0.182 0.415 0.33±0.07 0.32±0.087 0.838 1 230~1 250 0.382±0.088 0.324±0.166 0.705 0.257±0.086 0.204±0.068 0.262 1 380~1 420 0.381±0.084 0.394±0.121 0.516 0.278±0.072 0.299±0.074 0.632 1 430~1 470 0.377±0.082 0.416±0.11 0.255 0.282±0.061 0.328±0.074 0.284 1 500~1 600 0.663±0.073 0.691±0.095 0.29 0.588±0.065 0.583±0.095 0.917 1 600~1 700 0.965±0.057 0.886±0.101 0.274 0.896±0.076 0.739±0.134 0.058 2 910~2 950 0.417±0.057 0.406±0.056 0.788 0.54±0.045 0.511±0.02 0.158 2 950~2 990 0.386±0.074 0.388±0.049 0.955 0.515±0.051 0.485±0.018 0.182 3 300~3 500 0.775±0.163 0.862±0.118 0.497 0.999±0.001 0.996±0.002 0.015
Table 4. Spectral peak analysis of lung cancer cell lines
View tableView in ArticleTable 4. Spectral peak analysis of lung cancer cell lines
A549(Mean±SD) P-value SPCA(Mean±SD) P-value Control(n=12) SP(n=12) Control(n=12) SP(n=12) Range
/cm-11 070~1 090 1 082.166±2.172 1 079.121±2.713 0.021 1 081.871±4.421 1 080.996±5.535 0.468 1 230~1 250 1 235.728±3.219 1 231.871±3.049 0.018 1 232.353±3.993 1 232.835±4.581 0.516 1 380~1 420 1 397.004±1.767 1 398.209±3.571 0.329 1 397.004±3.082 1 397.486±3.409 0.626 1 430~1 470 1 451.245±2.305 1 453.655±5.716 0.38 1 451.486±3.409 1 450.796±1.767 0.782 1 500~1 600 1 545.021±4.991 1 534.655±5.899 0.001 1 546.468±3.779 1 535.619±3.049 0.001 1 600~1 700 1 651.092±3.779 1 637.351±8.117 <0.001 1 645.065±4.123 1 638.074±10.254 0.023 2 910~2 950 2 924.186±1.364 2 924.347±1.575 0.999 2 925.186±1.438 2 925.081±0.941 0.356 2 950~2 990 2 958.659±0.682 2 959.141±0.998 0.351 2 958.659±0.682 2 958.9±0.893 0.351 3 300~3 500 3 292.783±2.291 3 293.747±4.305 0.563 3 294.153±2.569 3 293.372±3.404 0.683
Table 5. Statistical analysis of spectral peak of lung cancer cell lines
View tableView in ArticleTable 5. Statistical analysis of spectral peak of lung cancer cell lines
A549(Mean±SD) P-value SPCA(Mean±SD) P-value Control(n=12) SP(n=12) Control(n=12) SP(n=12) Range
/cm-11 070~1 090 0.486±0.17 0.396±0.342 0.075 0.415±0.256 0.297±0.133 0.468 1 230~1 250 0.463±0.121 0.349±0.283 0.176 0.391±0.102 0.377±0.127 0.505 1 380~1 420 0.433±0.114 0.346±0.24 0.839 0.388±0.098 0.361±0.129 0.095 1 430~1 470 0.419±0.115 0.356±0.089 0.57 0.369±0.078 0.375±0.11 0.427 1 500~1 600 0.687±0.059 0.539±0.209 <0.001 0.656±0.083 0.644±0.153 0.583 1 600~1 700 0.951±0.018 0.714±0.267 <0.001 0.922±0.077 0.798±0.108 0.018 2 910~2 950 0.604±0.102 0.524±0.145 0.204 0.491±0.058 0.473±0.039 0.064 2 950~2 990 0.537±0.088 0.495±0.134 0.342 0.453±0.087 0.465±0.016 0.305 3 300~3 500 0.993±0.006 0.997±0.003 0.186 0.94±0.056 0.966±0.03 0.358
Tools
Get Citation
Copy Citation Text
Chao YANG, Zhen ZHU, Jing LI, Yun-heng SUN, Xue-yan OUYANG, Jia-wei WANG, Qian HUANG, Gang DING, Yao-sheng WANG, Feng JIANG. Changes of Tumor Cell Components Induced by Pain-Related Neuropeptides SP Investigated by Synchrotron Radiation Infrared Microscopic Imaging Technology[J]. Spectroscopy and Spectral Analysis, 2020, 40(11): 3579
Paper Information
Category: Research Articles
Received: Oct. 18, 2019
Accepted: --
Published Online: Jun. 18, 2021
The Author Email:
© Copyright 2018-2021 | Chinese Laser Press.
All Rights Reserved 沪ICP备15018463号-20