Photonics Research, Volume. 10, Issue 5, 1297(2022)
Polarization microwave-induced thermoacoustic imaging for quantitative characterization of deep biological tissue microstructures
Fig. 1. Polarization microwave thermoacoustic imaging (P-MTAI) principles. (a) The schematic of vectorial absorption-based P-MTAI. (b) TA signal amplitude in anisotropic targets as a function of
Fig. 2. P-MTAI system. (a) P-MTAI imaging experiment platform. (b) Photograph of the P-MTAI system. (c) Photograph of the linearly polarized microwave antenna.
Fig. 3. Simulated demonstration of polarization microwave thermoacoustic imaging (P-MTAI). (a) The schematic of sample parameters. The black and gray arrows represent microwave absorption coefficients parallel and perpendicular to the principal axis, respectively. (b) Simulated P-MTAI images of four samples with varying conductivity anisotropies. (c) DOMA images of four samples with varying conductivity anisotropy.
Fig. 4. Microwave polarization characterization. (a) Electrical conductivity of graphite absorber in different directions. (b) Schematic of the graphite absorber and the antenna at different angles (
Fig. 5. Resolution characterization. (a) Schematic of resolution experiment. (b) and (c) FWHM of the Gaussian-fitted lateral and axial profiles of the capillary tube.
Fig. 6. Verification of P-MTAI. (a) The photographs of the samples. The dotted black box represents a carbon fiber exhibiting anisotropic conductivity, and the dotted red box represents a carbon powder fiber with isotropic conductivity. (b) P-MTAI images for linearly polarized microwave excitation at varying orientations. (c) Statistical results for the TA signal amplitude shown in (b). (d) The DOMA image corresponding to (b). (e) The photographs of the samples after the application of H&E staining, including skeletal muscle tissue along the longitudinal section and cross section. (f) P-MTAI imaging of longitudinal section muscle and cross-sectional muscle excited by linearly polarized microwaves with differing polarization orientations. (g) Statistical results for the TA signal amplitude shown in (f). (h) The DOMA images corresponding to (f). (i) The P-MTAI images and DOMA images of carbon fibers at different depths. (j) The corresponding DOMA value of (d) at different depths.
Fig. 7. Application of P-MTAI. (a) Schematic of experimental device. (b) Schematic of the twisted spiral arrangement exhibited by cardiomyocytes. (c) The statistical analysis corresponding to the four regions of interest shown in (d). (d) Schematic of the heart from wild-type (WT, upper row) and myocardial infarction (MI, bottom row) rabbits wherein the red area marked by 4 is the region exhibiting MI. P-MTAI images of the heart were acquired for both types. The scale bar denotes 100 μm in the main micrographs and 10 mm otherwise. (e) Ultrasound images and corresponding DOMA images. (f) DOMA value statistics collected for different regions of the WT and MI images shown in (e). Each black dot (black circle, 1; black square, 2) represents a DOMA value for each individual WT myocardial region. Each red dot (red circle, 1; red square, 2) represents a DOMA value for each individual MI myocardial region. A total of
Fig. 8. Application of P-MTAI in deep tissue. (a) P-MTAI was applied to a rabbit heart covered with a layer of adipose tissue with a thickness of
Get Citation
Copy Citation Text
Yujing Li, Shanxiang Zhang, Linghua Wu, Zhongwen Cheng, Zhenhui Zhang, Haohao Wang, Shuxiang Zhao, Mingyang Ren, Sihua Yang, Da Xing, Huan Qin. Polarization microwave-induced thermoacoustic imaging for quantitative characterization of deep biological tissue microstructures[J]. Photonics Research, 2022, 10(5): 1297
Category: Imaging Systems, Microscopy, and Displays
Received: Jan. 7, 2022
Accepted: Mar. 15, 2022
Published Online: Apr. 27, 2022
The Author Email: Da Xing (xingda@scnu.edu.cn), Huan Qin (qinghuan@scnu.edu.cn)