Experiment Science and Technology, Volume. 22, Issue 3, 47(2024)

Design and Practice of the Comprehensive Experiment ofInduction and Detection of Autophagic Flux

Jiaoyan YANG1...2,3, Kaiyu LIU1,2,3, Jinju ZHANG1,2,3, Zequn WANG1,2,3, Bing LI1,2,3, and Yongbo YANG1,23,* |Show fewer author(s)
Author Affiliations
  • 1School of Life Sciences, Central China Normal University, Wuhan 430079, China
  • 2National-Level Virtual Simulation Experimental Teaching Center for Biology, Central China Normal University, Wuhan 430079, China
  • 3Hubei Province-level Experimental Teaching Demonstration Center of Biology, Central China Normal University, Wuhan 430079, China
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    Figures & Tables(5)
    • Table 1. [in Chinese]

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      Table 1. [in Chinese]

      内容现有实验本实验改进后的优点
      细胞传代与培养人宫颈癌细胞Hela细胞昆虫细胞昆虫细胞培养相对简单,传代采用机械吹打法, 过程无需胰酶消化
      细胞转染GFP-LC3单荧光标记mRFP-GFP-LC3 双荧光标记法既检测自噬现象,又能动态分析自噬流
      自噬诱导雷帕霉素处理饥饿处理昆虫细胞在饥饿条件下自噬现象非常明显,且显著 降低实验成本
    • Table 2. [in Chinese]

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      Table 2. [in Chinese]

      问卷内容非常必要/%必要/%一般/%不必要/%
      开设综合性实验45.052.52.50
      理论知识点理解和掌握50.046.33.70
      增强学习的兴趣60.032.57.50
      开阔视野57.542.500
      培养创新意识47.547.55.00
      提升分析和解决问题的能力51.245.03.80
      提升动手操作能力55.043.81.20
      培养科研思维能力51.346.22.50
      树立正确的“三观”57.537.53.81.2
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    Jiaoyan YANG, Kaiyu LIU, Jinju ZHANG, Zequn WANG, Bing LI, Yongbo YANG. Design and Practice of the Comprehensive Experiment ofInduction and Detection of Autophagic Flux[J]. Experiment Science and Technology, 2024, 22(3): 47

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    Paper Information

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    Received: Nov. 24, 2023

    Accepted: --

    Published Online: Jul. 8, 2024

    The Author Email: Yongbo YANG (杨勇波)

    DOI:10.12179/1672-4550.20230562

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