Experiment Science and Technology, Volume. 22, Issue 3, 47(2024)

Design and Practice of the Comprehensive Experiment ofInduction and Detection of Autophagic Flux

Jiaoyan YANG1...2,3, Kaiyu LIU1,2,3, Jinju ZHANG1,2,3, Zequn WANG1,2,3, Bing LI1,2,3, and Yongbo YANG1,23,* |Show fewer author(s)
Author Affiliations
  • 1School of Life Sciences, Central China Normal University, Wuhan 430079, China
  • 2National-Level Virtual Simulation Experimental Teaching Center for Biology, Central China Normal University, Wuhan 430079, China
  • 3Hubei Province-level Experimental Teaching Demonstration Center of Biology, Central China Normal University, Wuhan 430079, China
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    One of the important ways to improve the experimental teaching level and the talent training quality is dismantling, refining and adding the international frontier theories and technologies to the experimental teaching in time. The comprehensive experiment of induction and detection of autophagic flux is designed, which includes cell passage culture, cell transfection and induction and observation of autophagy. A systematic, comprehensive and exploratory modular experimental teaching content is formed. Through double fluorescent labeled autophagosome marker protein expressed in cells and starvation inducing, autophagosomes and autophagic flux are observed by the fluorescence microscope. The practice results in undergraduate experimental teaching indicate that it is helpful to cultivate students’ scientific research thinking and the innovative spirit, and improve their ability to analyze and solve problems.

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    Jiaoyan YANG, Kaiyu LIU, Jinju ZHANG, Zequn WANG, Bing LI, Yongbo YANG. Design and Practice of the Comprehensive Experiment ofInduction and Detection of Autophagic Flux[J]. Experiment Science and Technology, 2024, 22(3): 47

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    Paper Information

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    Received: Nov. 24, 2023

    Accepted: --

    Published Online: Jul. 8, 2024

    The Author Email: YANG Yongbo (杨勇波)

    DOI:10.12179/1672-4550.20230562

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