Acta Photonica Sinica, Volume. 49, Issue 9, 0918001(2020)

Progress and Prospect of Research on Single-molecule Localization Super-resolution Microscopy(Invited Review)

Sha AN1,2, Dan DAN1, Xiang-hua YU1, Tong PENG1,2, and Bao-li YAO1,2、*
Author Affiliations
  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
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    Super-resolution optical microscopy breaks through the diffraction limit and becomes a powerful tool for the modern biomedical research with the development of novel fluorescence probes, advanced lasers, high sensitivity photodetectors, etc. Single-molecule Localization Microscopy (SMLM), as one of the super-resolution technologies, can resolve the subcellular structures in nanoscale by using the photoswitching effect of certain fluorescence probes. In this paper, the principle and implementation of SMLM are introduced, the applications in the fields of cytobiology, tissue biology and neuroscience are presented, furthermore, the development trends and the futher investigated directions of this technique are discussed, providing references for the relevant research fields. The continuous innovation of super-resolution microscopy will promote the development of life science.

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    Sha AN, Dan DAN, Xiang-hua YU, Tong PENG, Bao-li YAO. Progress and Prospect of Research on Single-molecule Localization Super-resolution Microscopy(Invited Review)[J]. Acta Photonica Sinica, 2020, 49(9): 0918001

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    Paper Information

    Category: Microscopy

    Received: Jun. 4, 2020

    Accepted: Aug. 6, 2020

    Published Online: Dec. 26, 2020

    The Author Email: YAO Bao-li (yaobl@opt.ac.cn)

    DOI:10.3788/gzxb20204909.0918001

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