Laser & Optoelectronics Progress, Volume. 58, Issue 24, 2400006(2021)

Research Progress of Fluorescence Polarization Modulation Microscopy Imaging Technology

Zhibing Xu... Wenxia Zhou, Dongdong Xu, Xiao Wang, Jianhua Yin and Huijie Wang* |Show fewer author(s)
Author Affiliations
  • Department of Biomedical Engineering, College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu 211106, China
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    Figures & Tables(9)
    Absorption and radiative dipole moments of fluorescence dipole. (a) Dependence of fluorescence dipole absorption probability on angle between absorption dipole moment and electric field of exciting light; (b) emission intensity pattern of fluorescence dipole
    Schematic for measuring FA of molecules
    Schematic for measuring LD of molecules
    Geometrical models with different dipole orientation distributions obtained by TP-FPM method [26]
    Schematic of experiment setup of SPoD
    Images of hippocampal neurons (scale bar: 1 μm) [33]. (a) WF images; (b) SPoD images
    SDOM images of septin in live yeast cells (scale bar: 500 nm) [34]. (a) Orientation mapping images of septin with WF (upper-left) and SDOM (lower-right) under top view; (b) orientation mapping images under cross-sectional view
    Schematic of experiment setup of typical SIM
    pSIM imaging results of actin in BAPE cells (scale bar: 5 μm) [35]. (a) Intensity images by WF and SIM; (b) pseudocolored images in dipole direction by PM and pSIM; (c) magnified view of boxed region in Fig.9(a)
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    Zhibing Xu, Wenxia Zhou, Dongdong Xu, Xiao Wang, Jianhua Yin, Huijie Wang. Research Progress of Fluorescence Polarization Modulation Microscopy Imaging Technology[J]. Laser & Optoelectronics Progress, 2021, 58(24): 2400006

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    Paper Information

    Category: Reviews

    Received: Dec. 14, 2020

    Accepted: Mar. 3, 2021

    Published Online: Nov. 24, 2021

    The Author Email: Huijie Wang (wanghuijie@nuaa.edu.cn)

    DOI:10.3788/LOP202158.2400006

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