Quantitatively Experimental Study on Number of Viable Cells in Mixed Algae Based on Variable Fluorescence Statistical Analysis

Lu Wang; Gaofang Yin; Nanjing Zhao; Tingting Gan; Peilong Qi; Zhichao Ding; Xie Wang; Mingjun Ma; Ruifang Yang; Li Fang; Li Hu; Xiaoling Zhang

doi:10.3788/AOS202242.2401003

Acta Optica Sinica, Volume. 42, Issue 24, 2401003(2022)

Quantitatively Experimental Study on Number of Viable Cells in Mixed Algae Based on Variable Fluorescence Statistical Analysis

Lu Wang^1,2,3, Gaofang Yin^1,3、*, Nanjing Zhao^1,3, Tingting Gan^1,2,3, Peilong Qi^1,2,3, Zhichao Ding^1,2,3, Xie Wang^1,2,3, Mingjun Ma^1,3, Ruifang Yang^1,3, Li Fang^1,3, Li Hu⁴, and Xiaoling Zhang⁵

Author Affiliations

¹Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, China Academy of Sciences, Hefei 230031, Anhui , China

²University of Science and Technology of China, Hefei 230026, Anhui , China

³Key Laboratory of Environmental Optical Monitoring Technology of Anhui Province, Hefei 230031, Anhui , China

⁴School of Physics and Materials Engineering, Hefei Normal University, Hefei 230061, Anhui , China

⁵Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, Anhui , China

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Figures & Tables(10)

Fig. 1. Schematic diagram of experimental device for variable fluorescence measurement of viable algal cells in water

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Fig. 2. Comparison of viable algal cell density obtained by microscopic examination and measurement for seven mixed algal samples. (a) Selenastrum bibraianum and Scenedesmus Meyen;(b) Selenastrum bibraianum and Chlorella ellipsoidea; (c) Dunaliella salina and Amphidinium carterae Hulburt; (d) Heterosigma akashiwo and Prorocentrum lima; (e) Peridinium umbonatum and Scenedesmus Meyen; (f) Dunaliella salina and Chlorella sp.; (g) Peridinium umbonatum and Selenastrum bibraianum

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Fig. 3. Comparison of microscopic cell density of high SCVF algae and measured cell density in mixed algal samples. (a) Peridinium umbonatum and Scenedesmus Meyen; (b) Dunaliella salina and Chlorella sp.; (c) Peridinium umbonatum and Selenastrum bibraianum

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Fig. 4. Comparison of measurement results without and using filter separation method and microscopic results. (a) Mixed sample A; (b) mixed sample B; (c) mixed sample C; (d) mixed sample D

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Fig. 5. Comparison of microscopic algal cell density and measured algal cell density using filtration separation method. (a) Mixed sample A; (b) mixed sample B; (c) mixed sample C; (d) mixed sample D

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Table 0. [in Chinese]

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Table 0. [in Chinese]

Mixed algal sample	Parameter	Value
Peridinium umbonatum and Scenedesmus Meyen （SCVF ratio of 8.4）	Cell number proportion of Peridinium umbonatum	0	0.06	0.13	0.25	0.36	0.47	0.57	0.66	0.84	1.00
	Relative error absolute value /%	-	519	137	52	39	22	15	10	11	21
Dunaliella salina and Chlorella sp. （SCVF ratio of 9.4）	Cell number proportion of Dunaliella salina	0	0.11	0.22	0.33	0.43	0.63	0.82	0.91	1.00
Dunaliella salina and Chlorella sp. （SCVF ratio of 9.4）	Relative error absolute value /%	-	144	60	31	20	1	19	9	16
Peridinium umbonatum and Selenastrum bibraianum （SCVF ratio of 28.6）	Cell number proportion of Peridinium umbonatum	0	0.05	0.10	0.21	0.41	0.51	0.61	0.81	1.00
	Relative error absolute value /%	-	139	106	16	2	15	15	5	3

Table 1. Information of algal species used in experiment

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Table 1. Information of algal species used in experiment

Phylum	Species	Water area	Culture medium
Chlorophyta	Dunaliella salina	Ocean	F/2
	Scenedesmus Meyen	Freshwater	BG11
	Selenastrum bibraianum	Freshwater	BG11
	Chlorella ellipsoidea	Freshwater	BG11
	Chlorella pyrenoidosa	Freshwater	BG11
	Chlorella sp.	Ocean	F/2
Pyrrophyta	Peridinium umbonatum	Freshwater	119
	Heterosigma akashiwo	Ocean	F/2
	Amphidinium carterae Hulburt	Ocean	F/2
	Prorocentrum lima	Ocean	F/2

Table 2. Average of relative error absolute values of measured cell density with respect to microscopic cell density of seven mixed algae samples

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Table 2. Average of relative error absolute values of measured cell density with respect to microscopic cell density of seven mixed algae samples

Algal species composition of mixed samples	SCVF ratio of algae that make up mixed sample	Mean of absolute value of relative error /%
Selenastrum bibraianum and Scenedesmus Meyen	1.6	12
Selenastrum bibraianum and Chlorella ellipsoidea	2.1	7
Dunaliella salina and Amphidinium carterae Hulburt	2.6	8
Heterosigma akashiwo and Prorocentrum lima	5.3	17
Peridinium umbonatum and Scenedesmus Meyen	8.4	48
Dunaliella salina and Chlorella sp.	9.4	47
Peridinium umbonatum and Selenastrum bibraianum	28.6	59

Table 4. Algal species composition and SCVF ratio of four groups of mixed algal samples

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Table 4. Algal species composition and SCVF ratio of four groups of mixed algal samples

Sample label	Algal species composition of mixed samples	SCVF ratio of algae that make up mixed sample	Filter aperture /μm
A	Peridinium umbonatum and Chlorella pyrenoidosa （cultivating for 22 d）	39.8	5
B	Peridinium umbonatum and Chlorella pyrenoidosa （cultivating for 3 d）	67.5	5
C	Peridinium umbonatum and Selenastrum bibraianum	27.0	8
D	Peridinium umbonatum and Selenastrum bibraianum	31.0	5

Table 5. Mean of relative error absolute values of measured algal cell density before and after using filtration separation method with respect to microscopic algal cell density
View table
Table 5. Mean of relative error absolute values of measured algal cell density before and after using filtration separation method with respect to microscopic algal cell density
Sample label Result without filter separation method Result using filter separation method
A 58.4 5.5
B 60.0 10.0
C 43.8 8.0
D 50.0 7.4

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Lu Wang, Gaofang Yin, Nanjing Zhao, Tingting Gan, Peilong Qi, Zhichao Ding, Xie Wang, Mingjun Ma, Ruifang Yang, Li Fang, Li Hu, Xiaoling Zhang. Quantitatively Experimental Study on Number of Viable Cells in Mixed Algae Based on Variable Fluorescence Statistical Analysis[J]. Acta Optica Sinica, 2022, 42(24): 2401003

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Paper Information

Category: Atmospheric Optics and Oceanic Optics

Received: Apr. 27, 2022

Accepted: Jun. 13, 2022

Published Online: Dec. 14, 2022

The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn)

DOI:10.3788/AOS202242.2401003

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 0. [in Chinese]

Table 0. [in Chinese]

Table 1. Information of algal species used in experiment

Table 1. Information of algal species used in experiment

Table 2. Average of relative error absolute values of measured cell density with respect to microscopic cell density of seven mixed algae samples

Table 2. Average of relative error absolute values of measured cell density with respect to microscopic cell density of seven mixed algae samples

Table 4. Algal species composition and SCVF ratio of four groups of mixed algal samples

Table 4. Algal species composition and SCVF ratio of four groups of mixed algal samples

Table 5. Mean of relative error absolute values of measured algal cell density before and after using filtration separation method with respect to microscopic algal cell density

Table 5. Mean of relative error absolute values of measured algal cell density before and after using filtration separation method with respect to microscopic algal cell density