<trans-title xml:lang="en">Effects of Surface Wettability of Titanium Oxide Coatings on Osteoimmunomodulatory Properties</trans-title>

Li SHANGGUAN; Xiaoshuang NIE; Kuicai YE; Yuanyuan CUI; Yuqin QIAO

doi:10.15541/jim20230242

Journal of Inorganic Materials, Volume. 38, Issue 12, 1457(2023)

Effects of Surface Wettability of Titanium Oxide Coatings on Osteoimmunomodulatory Properties

Li SHANGGUAN^1,2, Xiaoshuang NIE^2,3, Kuicai YE^2,3, Yuanyuan CUI^1、*, and Yuqin QIAO^2,4、*

Author Affiliations

¹1. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China

²2. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China

³3. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

⁴4. School of Life Sciences, Shanghai University, Shanghai 200444, China

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Figures & Tables(10)

1. (a) Surface morphologies, (b) element compositions, (c) phase compositions, (d) electrochemical polarization curves, and (e) water contact angles of the samples

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2. Proliferation of Raw264.7 incubated on different samples for 1 and 4 d

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3. Morphologies of Raw264.7 cultured on different samples for 4 h, 1 d and 4 d

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4. Relative mRNA levels of inflammatory genes of Raw264.7 incubated on different samples for 4 d

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5. Cell proliferation of mBMSCs incubated on different samples for different time

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6. Cytoskeleton staining of mBMSCs incubated on different samples for 4, 12 and 24 h

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7. Relative mRNA levels of osteogenic genes of mBMSCs cultured on different samples for 10 d

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8. Relative mRNA levels of inflammatory genes of Raw264.7 co-cultured with mBMSCs on different samples for 3 d

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9. Relative mRNA levels of osteogenic genes of mBMSCs co-cultured with Raw264.7 on different samples for 3 d

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Table 1. Primers for real-time quantitative polymerase chain reaction (RT-qPCR)

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Table 1. Primers for real-time quantitative polymerase chain reaction (RT-qPCR)

Gene	Primer sequence (F: forward; R: Reverse; 5ʹ-3ʹ)
Arg1	F: GCC AGG GAC TGA CTA CCT TAAR: AGT TCT GTC TGC TTT GCT GTG
IL4	F: TCA TCC TGC TCT TCT TTC TCGR: CTT CTC CTG TGA CCT CGT TCA
CD206	F: AGG GAA GAG AAG AAG ATC CAGR: TGG GAG AAG ATG AAG TCA AAC
TNF-α	F: TAG CCA GGA GGG AGA ACA GAR: CCA GTG AGT GAA AGG GAC AGA
IL6	F: ACC AAG ACC ATC CAA TTC ATCR: CTG ACC ACA GTG AGG AAT GTC
CD86	F: TCT CCA ACA GCC TCT CTC TTTR: ATC TTC ATT GAC TCC GTT TCC
OCN	F: ACC GCC TAC AAA CGC ATC TAR: AGA GGA CAG GGA GGA TCA AGT
OPN	F: CTT GAG CAT TCC AAA GAG AGCR: CTT GTG GCT GTG AAA CTT GTG
BMP2	F: TAA GTT CTG TCC CCA GTG ACGR: TTC GGT GCT GGA AAC TAC TGT
TGF-β1	F: AAC CAA GGA GAC GGA ATA CAR: CGT GGA GTT TGT TAT CTT TGC
Runx2	F: GCA GCA CGC TAT TAA ATC CAAR: GCC AAA CAG ACT CAT CCA TTC

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Li SHANGGUAN, Xiaoshuang NIE, Kuicai YE, Yuanyuan CUI, Yuqin QIAO. Effects of Surface Wettability of Titanium Oxide Coatings on Osteoimmunomodulatory Properties[J]. Journal of Inorganic Materials, 2023, 38(12): 1457

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Paper Information

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Received: May. 19, 2023

Accepted: --

Published Online: Mar. 6, 2024

The Author Email: CUI Yuanyuan (cui-yy@shu.edu.cn), QIAO Yuqin (qiaoyq@mail.sic.ac.cn)

DOI:10.15541/jim20230242

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