Opto-Electronic Science, Volume. 2, Issue 11, 230037(2023)

Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging

Yuting Xiao1...2,4,†, Lianwei Chen1,2,3,4,†, Mingbo Pu1,2,3,4,*, Mingfeng Xu1,2,3,4, Qi Zhang1,2,3,4, Yinghui Guo1,2,3,4, Tianqu Chen1,2,4, and Xiangang Luo1,24,** |Show fewer author(s)
Author Affiliations
  • 1National Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, China
  • 2State Key Laboratory of Optical Technologies on Nano-Fabrication and Micro-Engineering, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
  • 3Research Center on Vector Optical Fields, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China
  • 4School of Optoelectronics, University of Chinese Academy of Sciences, Beijing 100049, China
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    Figures & Tables(5)
    (a) The SWM system configuration comprises four main components: a synthetic wave source generation module, a metalens, a pinhole, and a PMT. (b) The schematic diagram of the post-processing process. The first row is the four phase-shifting frames. The second row is the reconstructed quantitative phase gradient image. The third row is the reconstructed quantitative phase image.
    (a) The unit cell of the metalens is shown from both a top view and a side view. (b) The transmission coefficient's amplitude and (c) its phase delay for square lattice periodic nanopillar transmit arrays as a function of duty cycles of the nanopillars and the lattice constant. The square lattice period of 500 nm is indicated by a white dashed line. (d) The amplitude and phase of the transmission coefficient with a fixed lattice constant of 500 nm while varying the width of the nanopillars. (e) The calculated phase profile. (f) The schematic of metalens operating in transmission mode.
    (a) Normalized intensity distribution when metawave source is incident normally. The first row is 2D distribution. The dotted line indicates the Airy spot area. The second row is 1D distribution along the x- and y-direction. The arrow indicates the FWHM of focus spot. (b) Lateral resolution as a function of incident angles. (c) Normalized intensity profiles of the focal spot for different incident angles x-y plane. (d) Normalized intensity distribution along the x-direction in the x-y plane when metawave source is incident with different angles. The arrow represents the resolution of the SWM. The incident angles are 2.5°, 2°, 1.9° and 1.8°, respectively. D represents the distance between the centers of two focused light spots.
    (a) Normalized intensity curve recorded by PMT during one period for different phase differences. (b) The top section represents an anti-scattering imaging model with a medium screen positioned above the imaged object, while the bottom section depicts an anti-scattering imaging model with a medium screen placed below the imaged object. On the left-hand side, there is a map illustrating the distribution of the scattering media's phase. (c) Reconstruction phase difference vs. real phase difference. (d) Detection error as it varies with the distance of the scattering medium screen from the metalens, with the medium screen located above the imaged object. (e) Detection error as it varies with the distance of the scattering medium screen from the sample, with the medium screen situated below the imaged object.
    (a) LS-GLIM image and SWM image taken without the scattering layer. (b) traditional image taken with the scattering layer. (c) SWM image taken with the scattering layer.
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    Yuting Xiao, Lianwei Chen, Mingbo Pu, Mingfeng Xu, Qi Zhang, Yinghui Guo, Tianqu Chen, Xiangang Luo. Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging[J]. Opto-Electronic Science, 2023, 2(11): 230037

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    Paper Information

    Category: Research Articles

    Received: Oct. 7, 2023

    Accepted: Dec. 28, 2023

    Published Online: Mar. 19, 2024

    The Author Email: Pu Mingbo (MBPu), Luo Xiangang (XGLuo)

    DOI:10.29026/oes.2023.230037

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