This paper introduces a picometer measurement technology based on picometer comb. In the experiment, picometer comb is made by using twice exposed holography with a difference of 200 pm between the periods of two interference fields. When picometer comb is illuminated with a laser beam, it will produce interference fringes along the propagation. Since the period of the interference fringe is in reverse relation with the picometer difference of picometer comb, it implies a new principle of measuring the picometer displacement or variation. Based on the picometer measurement technique proposed in this paper, a new series of picometer-level optical elements, technique, and apparatus will be established in the near future.