Photonics Research, Volume. 9, Issue 8, 1477(2021)
Flexible, video-rate, and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing
Fig. 1. System configuration. The inset shows the enlarged view of the stepped mirror. The faint yellow plane represents the focal plane of O1. The positions of the stepped reflection surfaces were adjustable relative to the focal plane. Red lines and points represent two linear signals and their directions, respectively.
Fig. 2. Principle of stepped remote focusing and FOV-jointing. (a) Relative positions of the linear beams and the stepped mirror, as well as the geometric constraints of the distance between the linear beams and the step edge. Red, yellow, and pink points represent the linear beams perpendicular to the surface of the paper. (b) FOV-jointing module rearranges two parallel lines into a head-to-head line. The inset shows that the lateral interval between two linear signals is adjustable.
Fig. 3. Resolutions and Strehl ratio measurements in an axial range of
Fig. 4. Imaging the edges of the knife-edge mirrors of the FOV-jointing module and the stepped mirror. (a) Edges of KM1, KM2, KM01, and KM02 are shown clearly on the camera when they are conjugated and in focus. Scale bar, 50 μm. (b) Enlarged view of white square in (a). (c) Imaging the same area of (b) by moving KM01 10 μm down. Scale bar, 5 μm.
Fig. 5. Optical sectioning ability of the system. (a) and (b) Defocusing responses of thin fluorescent sheet in the line confocal mode of the two FOVs. (c) Line confocal optical sectioning thickness with a line width of 1 pixel. (d) Line confocal and (e) LiMo-reconstructed images of the same layer of a pollen grain at different remote focal positions in the two FOVs. Scale bar, 10 μm. Right column shows normalized intensity profiles along corresponding colored lines in the images.
Fig. 6. Video-rate imaging of two-layer Brownian motion of 200 nm fluorescent beads aqueous solution. (a) Sandwich structure of the sample. (b) Steps to track motion trajectories of the beads. (c) and (d) Trajectories of Brownian motions of the beads in FOV1 and FOV2. (e) and (f) are enlarged views of the areas indicated by the white squares in (c) and (d), respectively.
Fig. 7. Simultaneous dual-plane functional imaging of a transgenic zebrafish brain
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Rui Jin, Yalan Yu, Dan Shen, Qingming Luo, Hui Gong, Jing Yuan, "Flexible, video-rate, and aberration-compensated axial dual-line scanning imaging with field-of-view jointing and stepped remote focusing," Photonics Res. 9, 1477 (2021)
Category: Imaging Systems, Microscopy, and Displays
Received: Apr. 12, 2021
Accepted: Jun. 8, 2021
Published Online: Jul. 19, 2021
The Author Email: Hui Gong (huigong@mail.hust.edu.cn), Jing Yuan (yuanj@hust.edu.cn)