Acta Optica Sinica, Volume. 38, Issue 1, 0118001(2018)

Spot Array Optimization of Parallel Confocal Imaging Based on Digital Micromirror Device

Qian Zhu1,2,3, Xibin Yang2,3, Simin Li2, Hui Li2, Chi Wang1, Shoupeng Liu2, Junming Jian2, and Daxi Xiong3、*
Author Affiliations
  • 1 School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200072, China
  • 2 Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China
  • 3 Center of Optics Health, Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China
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    Figures & Tables(10)
    Optical path of parallel confocal imaging system based on DMD
    Parameters of DMD spot array. (a) Modulation of spot size; (b) modulation of spot interval (taking 2×2 micromirrors for example)
    DMD scanning mode of spot array with 1×1 spot size and two times of spot interval. (a) Traditional arrangement mode; (b) used arrangement mode; (c) used scanning mode
    Comparison of axial resolution with different spot sizes. (a) Depth response curves of different spot sizes; axial imaging depth of (b) 1×1, (c) 2×2 and (d) 3×3 spot sizes
    Comparison of axial resolution with different spot intervals. (a) 1×1 spot size; (b) 2×2 spot size; (c) 3×3 spot size
    Imaging of resolution plate with different spot arrays. (a) 1×1 spot size and two times of spot interval; (b)-(e) 1×1 spot size and two, four, six, eight times of spot interval; (f)-(i) 2×2 spot size and two, four, six, eight times of spot interval; (j)-(m) 3×3 spot size and two, four, six, eight times of spot interval
    (a) Vertical section line of “9-2” line pairs on resolution plate with spot size of 1×1 and T=4; (b) change of gray level of vertical section line in Fig. 7(a); (c) vertical section line of “8-5” line pairs on resolution plate with spot size of 2×2 and T=4; (d) change of gray level of vertical section line in Fig. 7(c); (e) vertical section line of “8-2”line pairs on resolution plate with spot size of 3×3 and T=4; (f) change of gray level of vertical section line in Fig. 7(e)
    Contrast of image with 1×1 spot size and different spot intervals
    Comparison of imaging speeds with different spot intervals
    Imaging of three-dimensional body grating. (a) Wide field imaging and (b) its intensity distribution; (c) parallel scanning imaging of optimal spot array and (d) its intensity distribution; (e) Leica confocal microscopy imaging and (f) its intensity distribution
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    Qian Zhu, Xibin Yang, Simin Li, Hui Li, Chi Wang, Shoupeng Liu, Junming Jian, Daxi Xiong. Spot Array Optimization of Parallel Confocal Imaging Based on Digital Micromirror Device[J]. Acta Optica Sinica, 2018, 38(1): 0118001

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    Paper Information

    Category: Microscopy

    Received: Jul. 28, 2017

    Accepted: --

    Published Online: Aug. 31, 2018

    The Author Email: Xiong Daxi (xiongdaxi@sibet.ac.cn)

    DOI:10.3788/AOS201838.0118001

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