Optics and Precision Engineering, Volume. 32, Issue 3, 324(2024)

Multi-wavelength lasers coupling system used in fluorescent microscopy

Minchao ZHOU1...2, Zhenya WANG2,*, Yi FANG2, Gangyin LUO2, Zhe ZHANG2 and Xiaojie SUN2 |Show fewer author(s)
Author Affiliations
  • 1Academy for Engineering and Technology, Fudan University, Shanghai200433, China
  • 2Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou15000, China
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    Figures & Tables(13)
    Optical fiber coupler
    Schematic of fiber coupling principle
    Design of coupling lens
    Comparison of chromatic focal shift of two lens models
    Decay curves of coupling efficiency with adjustment errors between coupling lens and fiber end face
    Structural of coupling component
    Assembly and adjustment of fiber coupling component
    Result of coupling lens test
    Assembly and adjustment of coupling devices
    • Table 1. Parameters of SM fiber

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      Table 1. Parameters of SM fiber

      Fiber modelPM-S405-XP
      Operating wavelength400~680 nm
      Core NA0.12
      Mode field diameter

      (3.3±0.5) μm@405 nm

      (4.6±0.5) μm@630 nm

      Cladding diameter(125.0±1.0) μm
      Core diameter3 μm
      Coating diameter(245±15) μm
      Core/clad offset≤0.6 μm
      Coating concentricity<5.0 μm
    • Table 2. Calculation result of coupling efficiency

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      Table 2. Calculation result of coupling efficiency

      Wavelength/nm

      X-axis light intensity

      distribution

      Y-axis light intensity distribution

      Within

      3.6 μm

      Within 4.8 μmWithin 3.6 μmWithin 4.8 μm
      40569816981
      48863756778
      56166766980
      63860606274
    • Table 3. Coupling efficiency test result

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      Table 3. Coupling efficiency test result

      Wavelength/nmPower before coupling/mWPower after coupling/mWCoupling efficiency/%
      40537.825.868.3
      48838.526.669.1
      56141.229.371.1
      63839.428.171.3
    • Table 4. Coupling efficiency test

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      Table 4. Coupling efficiency test

      Wavelength/nmPower before coupling/mWPower after coupling/mWCoupling Efficiency/%
      40554.035.365.4
      48853.335.065.7
      56153.637.269.4
      63851.234.567.4
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    Minchao ZHOU, Zhenya WANG, Yi FANG, Gangyin LUO, Zhe ZHANG, Xiaojie SUN. Multi-wavelength lasers coupling system used in fluorescent microscopy[J]. Optics and Precision Engineering, 2024, 32(3): 324

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    Paper Information

    Category:

    Received: Sep. 25, 2023

    Accepted: --

    Published Online: Apr. 2, 2024

    The Author Email: WANG Zhenya (wangzy@sibet.ac.cn)

    DOI:10.37188/OPE.20243203.0324

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