Spectroscopy and Spectral Analysis, Volume. 42, Issue 5, 1339(2022)
Research Progress of Pathogenic Bacteria and Their Drug Resistance Detection Based on Surface Enhanced Raman Technology
Fig. 2. Schematic diagram of bacterial aptamer-AgNPs formation in situ[23]
Fig. 3. (a) Agglutinin (Con A) functionalized silver-plated magnetic nanoparticles (Ag@MNPs) are combined with bacteria, and the bacteria are separated from the sample matrix in the presence of a magnet; (b) Antibody and SERS tag addition; (c) 3 AgNP conjugates are added together with 3 bacterial pathogens and Con A (combined with all three bacteria), and analyzed by 532 nm laser[25]; (d) AMP modified and The sample matrix (including bacteria, blood cells or other interfering substances) are cultured together; (e) Fe3O4NPs@bacterial complex is magnetically separated from the sample matrix; (f) 4-MPBA modified Au@Ag-GO is cultured with Fe3O4NPs@bacterial complex to form a sandwich structure[26]
Fig. 4. (a) Schematic diagram of the microfluidic system and device[27]; (b) Optical microscope image taken under 4X magnification showing the microfluidic channel and the active square at the center[28]; (c) The symmetrical arrangement of four electrodes with polynomial boundaries and the graphs of electric potential field distribution and electrophoretic force[30] ; (d) Schematic diagram of DEP-SERS device[29]
Fig. 5. (a) SEM image of S.aureus mixed with AgNPs- (left), AgNPs+ (right); (b) SERS spectra of S.aureus using AgNPs- or AgNPs+[32]
Get Citation
Copy Citation Text
Qiu-yue FU, Xiang-lin FANG, Yi ZHAO, Xun QIU, Peng WANG, Shao-xin LI. Research Progress of Pathogenic Bacteria and Their Drug Resistance Detection Based on Surface Enhanced Raman Technology[J]. Spectroscopy and Spectral Analysis, 2022, 42(5): 1339
Category: Research Articles
Received: Apr. 7, 2021
Accepted: --
Published Online: Nov. 10, 2022
The Author Email: FU Qiu-yue (fuqiuy@163.com)