Spectroscopy and Spectral Analysis, Volume. 42, Issue 5, 1339(2022)

Research Progress of Pathogenic Bacteria and Their Drug Resistance Detection Based on Surface Enhanced Raman Technology

Qiu-yue FU1,*... Xiang-lin FANG1,1;, Yi ZHAO2,2;, Xun QIU1,1;, Peng WANG1,1; and Shao-xin LI1,1; *; |Show fewer author(s)
Author Affiliations
  • 11. Biomedical Photonics Laboratory, School of Biomedical Engineering, Guangdong Medical University, Dongguan 523808, China
  • 22. Guangdong Provincial Key Laboratory of Molecular Diagnosis, Dongguan 523808, China
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    Figures & Tables(5)
    (a)—(d) High-magnification TEM images of Au@AgNPs with Ag shell thicknesses of 1, 3, 5, 7 nm respectively[18];(e) TEM images of Au@AgNR[16]; (f) SEM of B-g-PAMDAC[20]
    Schematic diagram of bacterial aptamer-AgNPs formation in situ[23]
    (a) Agglutinin (Con A) functionalized silver-plated magnetic nanoparticles (Ag@MNPs) are combined with bacteria, and the bacteria are separated from the sample matrix in the presence of a magnet; (b) Antibody and SERS tag addition; (c) 3 AgNP conjugates are added together with 3 bacterial pathogens and Con A (combined with all three bacteria), and analyzed by 532 nm laser[25]; (d) AMP modified and The sample matrix (including bacteria, blood cells or other interfering substances) are cultured together; (e) Fe3O4NPs@bacterial complex is magnetically separated from the sample matrix; (f) 4-MPBA modified Au@Ag-GO is cultured with Fe3O4NPs@bacterial complex to form a sandwich structure[26]
    (a) Schematic diagram of the microfluidic system and device[27]; (b) Optical microscope image taken under 4X magnification showing the microfluidic channel and the active square at the center[28]; (c) The symmetrical arrangement of four electrodes with polynomial boundaries and the graphs of electric potential field distribution and electrophoretic force[30] ; (d) Schematic diagram of DEP-SERS device[29]
    (a) SEM image of S.aureus mixed with AgNPs- (left), AgNPs+ (right); (b) SERS spectra of S.aureus using AgNPs- or AgNPs+[32]
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    Qiu-yue FU, Xiang-lin FANG, Yi ZHAO, Xun QIU, Peng WANG, Shao-xin LI. Research Progress of Pathogenic Bacteria and Their Drug Resistance Detection Based on Surface Enhanced Raman Technology[J]. Spectroscopy and Spectral Analysis, 2022, 42(5): 1339

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    Paper Information

    Category: Research Articles

    Received: Apr. 7, 2021

    Accepted: --

    Published Online: Nov. 10, 2022

    The Author Email: FU Qiu-yue (fuqiuy@163.com)

    DOI:10.3964/j.issn.1000-0593(2022)05-1339-07

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