Acta Optica Sinica (Online), Volume. 1, Issue 5, 0512001(2024)

Raman Determination of Low Mass Concentration Glucose Solutions Based on Internal Standard Method

Kang Du1,2, Yangyang Hua1,2, Shuaifei Wei1,2, Tingting Wang1,2, Guannan Qu1,2, Meng Zhao1,2, and Hongxing Cai1,2、*
Author Affiliations
  • 1School of Physics, Changchun University of Science and Technology, Changchun 130022, Jilin , China
  • 2Key Laboratory of Jilin Province for Spectral Detection Science and Technology, Changchun University of Science and Technology, Changchun 130022, Jilin , China
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    Figures & Tables(8)
    Experimental setup diagram of micro confocal Raman spectroscopy system
    Simulated Raman spectra of two glucopyranoses. (a) α-D-glucopyranose; (b) β-D-glucopyranose
    Raman spectra of glucose solution with different mass concentrations
    Spectra of glucose solution normalized with water as internal standard
    Linear relationship between glucose solution mass concentration and relative intensity of characteristic peaks
    Linear relationship between glucose solution mass concentration and relative area of characteristic peaks
    • Table 1. Relative intensity of characteristic peaks of glucose solution

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      Table 1. Relative intensity of characteristic peaks of glucose solution

      Glucose

      mass concentration /(mg·dL-1

      Relative intensityAverage relative intensity RIStandard deviation DI
      R1R2R3
      1600.00.291310.311060.306240.302870.01031
      800.00.194130.172540.184490.183720.01082
      400.00.122240.103290.112910.112810.00948
      200.00.071960.068660.070110.070240.00165
      100.00.059450.051340.055680.055490.00406
      50.00.037490.042690.042040.040740.00283
      25.00.027490.038360.032040.032630.00054
      12.50.025960.026190.024810.025650.00074
    • Table 2. Relative area of characteristic peaks in glucose solution

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      Table 2. Relative area of characteristic peaks in glucose solution

      Glucose

      mass concentration /(mg·dL-1

      Relative areaAverage relative area SAStandard deviation DA
      S1S2S3
      1600.00.103610.093480.104740.100610.00621
      800.00.070170.067870.060770.066270.00492
      400.00.040210.043970.044460.042880.00233
      200.00.030870.027610.026870.028450.00213
      100.00.026730.024980.023620.025110.00156
      50.00.023010.021780.015690.020160.00392
      25.00.016740.018270.017250.017420.00077
      12.50.011670.013490.012490.012550.00091
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    Kang Du, Yangyang Hua, Shuaifei Wei, Tingting Wang, Guannan Qu, Meng Zhao, Hongxing Cai. Raman Determination of Low Mass Concentration Glucose Solutions Based on Internal Standard Method[J]. Acta Optica Sinica (Online), 2024, 1(5): 0512001

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    Paper Information

    Category: Research Articles

    Received: Jul. 18, 2024

    Accepted: Sep. 12, 2024

    Published Online: Dec. 17, 2024

    The Author Email: Cai Hongxing (caihx@cust.edu.com)

    DOI:10.3788/AOSOL240441

    CSTR:32394.14.AOSOL240441

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