Multifocus Microscopic Image Fusion Algorithm

Hongyu Fu; Yan Gong; Luhan Wang; Yanwei Zhang; Song Lang; Zhi Zhang; Hanqing Zheng

doi:10.3788/LOP232015

Laser & Optoelectronics Progress, Volume. 61, Issue 6, 0618022(2024)

Multifocus Microscopic Image Fusion Algorithm

Hongyu Fu^1,2, Yan Gong^1,2、*, Luhan Wang², Yanwei Zhang², Song Lang², Zhi Zhang², and Hanqing Zheng²

¹Division of Life Sciences and Medicine, School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Suzhou 215163, Jiangsu , China

²Medical Optical Research Laboratory, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, Jiangsu , China

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Figures & Tables(10)

Fig. 1. Flowchart of the proposed algorithm

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Fig. 2. Photograph of wide-field-of-view high-resolution microscope system^[19]

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Fig. 3. Multifocus microscopic image sequence of ant. (a)‒(j) The 1st to the 10th ant-mounted microscopic images

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Fig. 4. Multifocus microscopic image sequence of pogonatum. (a)‒(j) The 1st to the 10th pogonatum-mounted microscopic images

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Fig. 5. Multifocus microscopic image sequence of charophyte. (a)‒(m) The 1st to the 13th charophyte-mounted microscopic images

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Fig. 6. Comparison of fusion results among different methods. (a) Ant fusion results; (b) pogonatum fusion results; (c) charophyte fusion results

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Fig. 7. Comparison of local magnification results among different methods. (a) Local magnification results of ant; (b) local magnification results of pogonatum; (c) local magnification results of charophyte

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Table 1. Comparison of evaluation indexes of different methods for ant

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Table 1. Comparison of evaluation indexes of different methods for ant

Method	STD	PSNR	SSIM	MI	Q_G
RP	35.6760	77.3979	0.8883	3.7610	0.1849
DWT	35.8452	77.2162	0.8901	3.6081	0.1858
NSCT	35.7871	77.3761	0.9230	3.8244	0.2069
MWGF	36.0806	76.8592	0.8720	3.7815	0.1528
DCT-SVD	35.4951	77.5662	0.9230	3.9352	0.1470
Proposed method	38.3836	79.7696	0.9440	4.3580	0.1878

Table 2. Comparison of evaluation indexes of different methods for pogonatum

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Table 2. Comparison of evaluation indexes of different methods for pogonatum

Method	STD	PSNR	SSIM	MI	Q_G
RP	37.0346	75.2809	0.8830	2.6074	0.1812
DWT	37.1449	75.1473	0.8839	2.4331	0.1837
NSCT	37.1040	75.2381	0.8819	2.6639	0.2054
MWGF	39.0683	76.6151	0.9051	2.7743	0.1577
DCT-SVD	36.1581	75.5040	0.9064	2.7331	0.1491
Proposed method	41.5193	77.9923	0.9403	2.8720	0.1774

Table 3. Comparison of evaluation indexes of different methods for charophyte

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Table 3. Comparison of evaluation indexes of different methods for charophyte

Method	STD	PSNR	SSIM	MI	Q_G
RP	24.0237	81.3083	0.8938	3.5964	0.2059
DWT	24.1885	81.0245	0.8940	3.4569	0.2013
NSCT	24.2104	81.1230	0.8912	3.6181	0.2305
MWGF	24.7625	83.6119	0.9424	3.9624	0.1757
DCT-SVD	24.8516	82.7793	0.9346	3.9138	0.1786
Proposed method	25.2177	84.4794	0.9498	4.1692	0.2206

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Hongyu Fu, Yan Gong, Luhan Wang, Yanwei Zhang, Song Lang, Zhi Zhang, Hanqing Zheng. Multifocus Microscopic Image Fusion Algorithm[J]. Laser & Optoelectronics Progress, 2024, 61(6): 0618022

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Paper Information

Category: Microscopy

Received: Aug. 31, 2023

Accepted: Oct. 13, 2023

Published Online: Mar. 29, 2024

The Author Email: Gong Yan (gongy@sibet.ac.cn)

DOI:10.3788/LOP232015

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology