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Acta Optica Sinica, Volume. 42, Issue 6, 0617001(2022)

Analytical Method of Viable Algae Cells in Water Based on Variable Fluorescence Statistical Distribution

Lu Wang1,2,3, Gaofang Yin1,3、*, Nanjing Zhao1,3、**, Tingting Gan1,2,3, Peilong Qi1,2,3, Zhichao Ding1,2,3, Hui Hua1,2,3, Min Chen1,2,3, Mingjun Ma1,3, Ruifang Yang1,3, and Li Fang1,3
Author Affiliations
  • 1Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optic and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China
  • 2University of Science and Technology of China, Hefei, Anhui 230026, China
  • 3Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei, Anhui 230031, China
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    Figures & Tables(10)
    Distribution shape of cell number and Fv value under different cell density. (a) Distribution shape of cell number; (b) SCVF is 0.001, distribution shape of Fv value; (c) SCVF is 0.010, distribution shape of Fv value

    Fig. 1. Distribution shape of cell number and Fv value under different cell density. (a) Distribution shape of cell number; (b) SCVF is 0.001, distribution shape of Fv value; (c) SCVF is 0.010, distribution shape of Fv value

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    Distribution shape of Fv value of random sample

    Fig. 2. Distribution shape of Fv value of random sample

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    Schematic of algae variable fluorescence measurement experimental system for random sampling of uniform samples

    Fig. 3. Schematic of algae variable fluorescence measurement experimental system for random sampling of uniform samples

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    Variation curves of mean Fv of subsamples with viable algae cell density. (a) Peridinium umbonatum var. inaequale; (b) Dunaliella salina; (c) Thalassiosira weissflogii; (d) Prorocentrum lima; (e) Chlorella sp.

    Fig. 4. Variation curves of mean Fv of subsamples with viable algae cell density. (a) Peridinium umbonatum var. inaequale; (b) Dunaliella salina; (c) Thalassiosira weissflogii; (d) Prorocentrum lima; (e) Chlorella sp.

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    Comparison of viable algal cell density between microscopic examination and statistical analysis method. (a) Peridinium umbonatum var. inaequale; (b) Dunaliella salina; (c) Thalassiosira weissflogii; (d) Prorocentrum lima; (e) Chlorella sp.

    Fig. 5. Comparison of viable algal cell density between microscopic examination and statistical analysis method. (a) Peridinium umbonatum var. inaequale; (b) Dunaliella salina; (c) Thalassiosira weissflogii; (d) Prorocentrum lima; (e) Chlorella sp.

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    Variation curves of mean Fv of subsamples with viable algae cell density under different extended culture days. (a) 3 days; (b) 6 days; (c) 9 days; (d) 12 days; (e) 15 days; (f) 18 days; (g) 23 days; (h) 28 days; (i) 33 days

    Fig. 6. Variation curves of mean Fv of subsamples with viable algae cell density under different extended culture days. (a) 3 days; (b) 6 days; (c) 9 days; (d) 12 days; (e) 15 days; (f) 18 days; (g) 23 days; (h) 28 days; (i) 33 days

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    Relationship between SCVF and cell density of algal cells, and culture days. (a) SCVF; (b) cell desity

    Fig. 7. Relationship between SCVF and cell density of algal cells, and culture days. (a) SCVF; (b) cell desity

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    Comparison between microscopic observation and statistical analysis of viable algae cell density under different extended culture days. (a) 3 days; (b) 6 days; (c) 9 days; (d) 12 days; (e) 15 days; (f) 18 days; (g) 23 days; (h) 28 days; (i) 33 days

    Fig. 8. Comparison between microscopic observation and statistical analysis of viable algae cell density under different extended culture days. (a) 3 days; (b) 6 days; (c) 9 days; (d) 12 days; (e) 15 days; (f) 18 days; (g) 23 days; (h) 28 days; (i) 33 days

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    • Table 1. Average value of absolute value of relative error between calculated density and microscopic density of 5 kinds of algae samples

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      Table 1. Average value of absolute value of relative error between calculated density and microscopic density of 5 kinds of algae samples

      SpeciesAverage valueof absolute valueof relative error /%
      Peridinium umbonatum var. inaequale7.90
      Dunaliella salina9.11
      Thalassiosira weissflogii9.27
      Prorocentrum lima5.98
      Chlorella sp.14.89

    • Table 2. Average value of absolute value of relative error of calculated density with respect to microscopic density under different culture days

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      Table 2. Average value of absolute value of relative error of calculated density with respect to microscopic density under different culture days

      Algae culture days369121518232833
      Average value of absolutevalue of relative error /%10.566.558.099.819.1116.949.559.278.94
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    Lu Wang, Gaofang Yin, Nanjing Zhao, Tingting Gan, Peilong Qi, Zhichao Ding, Hui Hua, Min Chen, Mingjun Ma, Ruifang Yang, Li Fang. Analytical Method of Viable Algae Cells in Water Based on Variable Fluorescence Statistical Distribution[J]. Acta Optica Sinica, 2022, 42(6): 0617001

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    Paper Information

    Category: Medical optics and biotechnology

    Received: Aug. 31, 2021

    Accepted: Oct. 8, 2021

    Published Online: Mar. 8, 2022

    The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn), Zhao Nanjing (njzhao@aiofm.ac.cn)

    DOI:10.3788/AOS202242.0617001

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology