Acta Photonica Sinica, Volume. 51, Issue 8, 0851517(2022)
Advances in Multicolor Single-molecule Localization Microscopy（Invited）
Figures & Tables(7)
Fig. 1. Schematic diagram of the imaging principle of Exchange-PAINT［29］
Fig. 2. Performance of globLoc on ratiometric multi-color data. 4 color 3D imaging of Nup62-DY634，Nup96-AF647，ELYS-CF660C and WGA-CF680 in the NPC［40］
Fig. 3. Dual-color imaging of microtubules and mitochondria using grating［42］
Fig. 5. The optical system and Representation of the phase masks placed at the pupil plane［64］
Table 1. Dye combinations used in different multicolor SMLM
View tableView in ArticleTable 1. Dye combinations used in different multicolor SMLM
Fluorophore Target Type References Atto 520，AF647 538 nm，665 nm NPC More excitation ［ 20 ］mEos2，AF647 519 nm，665 nm Plasma membrane ［ 21 ］Dronpa，AF647 518 nm，665 nm HIV-1 virions ［ 22 ，23 ］AF405-Cy5，Cy2-Cy5，Cy3-Cy5 421 nm，504 nm
556 nm
Microtubules and clathrin-coated pits，m-RNA Activator-reporter ［
25 ］［
27 ］AF405-AF647 665 nm Microtubules and mitochondrial outer membrane protein Quenching ［ 28 ］AF488，AF514，Cy3 519 nm，543 nm
568 nm
F-actin，clathrin，and tubulin Ratiometric SMLM ［ 33 ］AF647，DY634，CF660C，CF680 665 nm，658 nm
685 nm，698 nm
Nup62，Nup96，ELYS and WGA in the NPC ［ 40 ］Dy634，DL650，CF660C，CF680 658 nm，673 nm
685 nm，698 nm
Peroxisomes，vimentin filaments，microtubules and the outer mitochondrial membrane SR-STORM ［ 41 ］
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Yuehan ZHAO, Xiang HAO. Advances in Multicolor Single-molecule Localization Microscopy（Invited）[J]. Acta Photonica Sinica, 2022, 51(8): 0851517
Paper Information
Category: Special Issue for the 60th Anniversary of XIOPM of CAS, and the 50th Anniversary of the Acta Photonica Sinica Ⅱ
Received: Jun. 3, 2022
Accepted: Jul. 27, 2022
Published Online: Oct. 25, 2022
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