Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment

Guang Han; Jingjing Gao; Mengru Tu; Jia Shi; Zhenhua Pan; Hongli Chen

doi:10.3788/LOP202158.0917001

Laser & Optoelectronics Progress, Volume. 58, Issue 9, 0917001(2021)

Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment

Guang Han^1,3, Jingjing Gao¹, Mengru Tu¹, Jia Shi^2,3, Zhenhua Pan⁴, and Hongli Chen^1,3、*

Author Affiliations

¹School of Life Sciences, Tiangong University, Tianjin 300387, China

²School of Electronic and Information Engineering, Tiangong University, Tianjin 300387, China

³Tianjin Key Laboratory of Photoelectric Detection Technology and Systems, Tianjin 300387, China

⁴Institute of Lung Cancer, General Hospital of Tianjin Medical University, Tianjin 300052, China

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Figures & Tables(10)

Fig. 1. Cell morphology observation results. （a） Normal medium; （b） high glucose medium

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Fig. 2. Absorbance of CCC-ESFs irradiated by 808 nm laser with different power densities (n=3;* means p<0.05, compared with high glucose control group)

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Fig. 3. Absorbance of CCC-ESFs irradiated by 808 nm laser with different energy densities (n=3; * means p<0.05, compared with high glucose control group;** means p<0.01, compared with high glucose control group)

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Fig. 4. Intracellular ROS level (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group; ## means p<0.01, compared with high glucose control group)

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Fig. 5. Total intracellular SOD level (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group)

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Fig. 6. TAC level of cells (n=3; * means p<0.05, compared with normal glucose control group; ** means p<0.01, compared with normal glucose control group; # means p<0.05, compared with high glucose control group; ## means p<0.01, compared with high glucose control group)

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Fig. 7. Intracellular mitochondrial membrane potential level

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Table 1. Irradiation parameters
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Table 1. Irradiation parameters
Parameter Value
Wavelength /nm 808
Output mode Continuous
Average radiant power /mW 40
Spot area /cm² 2
Vertical distance /cm 4
Beam profile Round
Beam divergence angle /(°) 90

Table 2. Laser irradiation dose
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Table 2. Laser irradiation dose
Power density /(mW·cm^-2) Energy density /(J·cm^-2) Irradiation time /min
10 1.5 2.5
3 5
6 10
12 20
20 1.5 1.25
3 2.5
6 5
12 10
40 1.5 0.625
3 1.25
6 2.5
12 5

Table 3. NF-κB, TNF-α and IL-1β levels of cells irradiated by 808 nm laser

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Table 3. NF-κB, TNF-α and IL-1β levels of cells irradiated by 808 nm laser

Item	NF-κB level /（pg·mL^-1）		TNF-α level /（pg·mL^-1）		IL-1β level /（pg·mL^-1）
Item	A₄₅₀	Standard deviation	A₄₅₀	Standard deviation	A₄₅₀	Standard deviation
Control-HG	1066.22	±6.81	74.11	±0.55	71.33	±0.55
Control-NG	1171.22	±10.86*	78.23	±0.37*	74.92	±0.15**
3 J·cm^-2-HG	1061.22	±66.35	75.72	±2.26**	66.48	±0.79**
3 J·cm^-2-NG	1111.22	±36.8^##	74.45	±0.96^#	69.72	±1.16^##

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Guang Han, Jingjing Gao, Mengru Tu, Jia Shi, Zhenhua Pan, Hongli Chen. Effect of Photobiomodulation Mediated by 808 nm Laser on Active Oxygen Steady-State in CCC-ESFs under High Glucose Environment[J]. Laser & Optoelectronics Progress, 2021, 58(9): 0917001

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