Journal of Innovative Optical Health Sciences, Volume. 14, Issue 3, 2140012(2021)
In vivo evaluation of laser-induced choroidal neovascularization in rats simultaneously using optical coherence tomography and photoacoustic microscopy
Determination of the precise location and the degree of the Choroidal neovascularization (CNV) lesion is essential for diagnosation Neovascular age-related macular degeneration (AMD) and evaluation the efficacy of treatment. Noninvasive imaging techniques with specific contrast for CNV evaluation are demanded. In this paper, two noninvasive imaging techniques, namely Optical coherence tomography (OCT) and Photoacoustic microscopy (PAM), are combined to provide specific detection of CNV for their complimentary contrast mechanisms. In vivo timeserial evaluation of Laser-induced CNV in rats is present at days 1, 3, 5, 7, 14, 21 after laser photocoagulation is applied to the rat fundus. Both OCT and PAM show that the CNV increases to its maximum at day 7 and decreases at day 14. Quantification of CNV area and CNV thickness is given. The dual-modal information of CNV is consistent with the histologic evaluation by hematoxylin and eosin (H&E) staining.
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Fengxian Du, Lei Gao, Lin Li, Qian Li, Fenghua Wang, Chuanqing Zhou, Cuixia Dai. In vivo evaluation of laser-induced choroidal neovascularization in rats simultaneously using optical coherence tomography and photoacoustic microscopy[J]. Journal of Innovative Optical Health Sciences, 2021, 14(3): 2140012
Received: Sep. 30, 2020
Accepted: Jan. 26, 2021
Published Online: Aug. 6, 2021
The Author Email: Zhou Chuanqing (zhoucq@sjtu.edu.cn)