A novel biochemcal sensor based on a high-Field Asymmetric Ion Mobility Spectrometry(FAIMS) theory is fabricated and characterized,which is mainly composed of an ionization source,a drift tube and a detector. By taking a VUV lamp as the ionization source and choosing an acetone with nitrogen (99.999%) as the carrier gas,the biochemical sensor works at ambient pressure.Moreover,two kinds of drift tubes consisting of two parallel plate electrodes are fabricated. One drift tube with the dimension of 25 mm×10 mm×1 mm is composed of two copper planar electrodes, the other with the dimension of 20 mm×10 mm×1.5 mm is fabricated by Micro Electro-Mechanical System(MEMS ) technology. For the former,the gap between the two drift tubes is 1 mm and the electrical field is above 10 000 V/cm;but for the latter,the gap between MEMS drift tube is 1.5 mm and the electrical field is less than 10 000 V/cm,which can not separate the acctone. With a peak-peak of rectangular asymmetric waveform voltage up to 1 600 V,a frequency of 195.8 kHz,a duty cycle of 30% and a compenstaion voltage of -8.63 V,the acetone displacement is compensated and the sensor ability to separate ions is demonstrated by an experiment using the planar copper drift tube.