Photonics Insights, Volume. 4, Issue 1, R01(2025)

High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications

Han Wang1,2、†, Wenshu Wang1,2, Xinzhu Xu1,2, Meiqi Li2,3、*, and Peng Xi1,2、*
Author Affiliations
  • 1College of Future Technology, Peking University, Beijing, China
  • 2National Biomedical Imaging Center, Peking University, Beijing, China
  • 3School of Life Sciences, Peking University, Beijing, China
  • show less
    References(152)

    [5] E. Abbe. Contributions to the theory of the microscope and the nature of microscopic vision. Selected Papers on Resolution Enhancement Techniques in Optical Lithography, Ed. F.M. Schellenberg, SPIE Milestone Series, 178, 12(2004).

    [23] T. Wöllert, G. M. Langford. Super-resolution imaging of the actin cytoskeleton in living cells using TIRF-SIM. Cytoskeleton: Methods and Protocols, 3(2022).

    [32] C. R. Sheppard. Super-resolution in confocal imaging. Optik, 80, 53(1988).

    [41] X. Wu, J. A. Hammer. Confocal Microscopy: Methods and Protocols, 111(2021).

    [60] G. Holst. Scientific CMOS camera technology: a breeding ground for new microscopy techniques. Microscopy and Analysis(2014).

    [85] J. Huff, A. Bergter, B. Luebbers. Multiplex mode for the LSM 9 series with Airyscan 2: fast and gentle confocal super-resolution in large volumes. Nat. Methods, 10, 1(2019).

    [121] S. Wischnitzer. Introduction to Electron Microscopy(2013).

    Tools

    Get Citation

    Copy Citation Text

    Han Wang, Wenshu Wang, Xinzhu Xu, Meiqi Li, Peng Xi, "High-spatiotemporal-resolution structured illumination microscopy: principles, instrumentation, and applications," Photon. Insights 4, R01 (2025)

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Review

    Received: Dec. 11, 2024

    Accepted: Feb. 4, 2025

    Published Online: Mar. 26, 2025

    The Author Email: Li Meiqi (limeiqi@pku.edu.cn), Xi Peng (xipeng@pku.edu.cn)

    DOI:10.3788/PI.2025.R01

    CSTR:32396.14.PI.2025.R01

    Topics