Spectroscopy and Spectral Analysis, Volume. 42, Issue 2, 642(2022)
The Removal of Water Peaks From IR Spectra of BSA Aqueous Solution by Two ATR Background Samples and Thermal Behavior of Proteins
Fig. 1. Schematic diagram of the removing of water peaks in BSA aqueous solution by two background samples
Fig. 2. Infrared spectra of 50 mg·mL-1 BSA aqueous solutions
Air ATR 32 scans plus water ATR:
Fig. 3. Comparison between the spectral subtraction and two background samples method
Fig. 4. IR spectra of BSA aqueous solutions obtained by two background samples
Heat treatment temperature: (a): 25 ℃; (b): 40 ℃; (c): 55 ℃; (d): 70 ℃; (e): 85 ℃
Fig. 5. IR spectra of BSA aqueous solutions after the removing of water peaks
Heat treatment temperature:
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Yang-biao XU, Hai-shui WANG. The Removal of Water Peaks From IR Spectra of BSA Aqueous Solution by Two ATR Background Samples and Thermal Behavior of Proteins[J]. Spectroscopy and Spectral Analysis, 2022, 42(2): 642
Category: Research Articles
Received: Apr. 1, 2021
Accepted: Nov. 16, 2021
Published Online: Apr. 2, 2022
The Author Email: XU Yang-biao (2393165519@qq.com)