Chinese Journal of Quantum Electronics, Volume. 40, Issue 6, 827(2023)
Study on interaction between taurine and human serum protein by spectroscopy
Figures & Tables(8)
Fig. 1. Fluorescence spectra of taurine and HSA at different temperatures
Fig. 2. Stern-Volmer curve of fluorescence quenching of taurine and HSA at different temperatures
Fig. 3. Double logarithmic curve of taurine-HSA system at different temperatures
Fig. 5. The fluorescence spectrum of HSA and the UV spectrum of taurine
Table 1. Stern⁃Volmer constant of taurine⁃HSA system
View tableView in ArticleTable 1. Stern⁃Volmer constant of taurine⁃HSA system
T / K Ksv/M-1 Kq/(M-1s-1) R2 291 3.28×103 1.24×1011 0.9548 301 3.01×103 3.01×1011 0.94571 311 2.69×103 2.69×1011 0.97733
Table 2. The binding constant and number of binding sites of taurine and HSA
View tableView in ArticleTable 2. The binding constant and number of binding sites of taurine and HSA
T / K Ka Ka n R2 291 1.57547 ± 0.11638 37.6 0.50435 ± 0.0247 0.9743 301 1.8267 ± 0.14185 66.9 0.57197 ± 0.0301 0.97043 311 2.4619 ± 0.20787 260 0.74448 ± 0.04412 0.96281
Table 3. The Stern⁃Volmer logarithm equation of taurine⁃HSA system combinedwith constant Ka and related thermodynamic parameters
View tableView in ArticleTable 3. The Stern⁃Volmer logarithm equation of taurine⁃HSA system combinedwith constant Ka and related thermodynamic parameters
T/K Kb △H/(k∙J∙mol-1) ΔS/(J∙mol-1∙K-1) △G/(k∙J∙mol-1) 291 37.6 -8707.6336 33.41395 -1.8431×104 301 66.9 -8707.6336 33.41395 -1.8765×104 311 260 -8707.6336 33.41395 -1.9099×104
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Ye YU, Juan LIAO, Bin WEN, Xin DENG. Study on interaction between taurine and human serum protein by spectroscopy[J]. Chinese Journal of Quantum Electronics, 2023, 40(6): 827
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Received: Jan. 22, 2022
Accepted: --
Published Online: Dec. 22, 2023
The Author Email: DENG Xin (260446391@qq.com)
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