Spectroscopy and Spectral Analysis, Volume. 40, Issue 12, 3748(2020)

Characterization and Effects of pH on the Conformation of Hemp Protein Isolate Based on Multi-Spectroscopic Technique

Jian-hua ZENG*, Yan MENG, Lin-lin LIU, Yang YANG, Mei-ying LI, Zi-yue WANG, Xiu-qing ZHU, and Yan-guo SHI
Author Affiliations
  • Key Laboratory of Grain Food and Comprehensive Processing of Heilongjiang Province, College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
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    Figures & Tables(8)
    SDS-PAGE of HPI
    Size (a) and zeta-potential profiles (b) of HPI and SPI
    Circular dichroism (a), FTIR (b) and curve-fitting results of amide I band (c: HPI, d: SPI) of HPI and SPI
    Intrinsic fluorescence (a) and Ultraviolet spectrum (b). The illustration is ultravidet second derivative spectrum of HPI and SPI
    Effects of different pH on the intrinsic fluorescence of HPI
    Effects of different pH on the ultraviolet spectrum of HPI
    Effects of different pH on the size of HPI
    • Table 1. Free sulfydryl tatol sulfydryl and disulfide bond content in HPI and SPI,as well as the value of Free sulfydryl/tatol sulfydryl

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      Table 1. Free sulfydryl tatol sulfydryl and disulfide bond content in HPI and SPI,as well as the value of Free sulfydryl/tatol sulfydryl

      ProteinFree sulfydryl
      /(μmol·g-1)
      Total free sulfydryl
      /(μmol·g-1)
      Disulfide bond
      /(μmol·g-1)
      Free sulfydryl/
      Total free sulfydryl
      HPI12.14±0.56a32.26±1.45a10.06±0.34a0.38b
      SPI4.21±0.25b9.51±0.45b2.66±0.11b0.44a
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    Jian-hua ZENG, Yan MENG, Lin-lin LIU, Yang YANG, Mei-ying LI, Zi-yue WANG, Xiu-qing ZHU, Yan-guo SHI. Characterization and Effects of pH on the Conformation of Hemp Protein Isolate Based on Multi-Spectroscopic Technique[J]. Spectroscopy and Spectral Analysis, 2020, 40(12): 3748

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    Paper Information

    Category: Research Articles

    Received: Oct. 31, 2019

    Accepted: --

    Published Online: Jun. 18, 2021

    The Author Email: ZENG Jian-hua (foodleslie@163.com)

    DOI:10.3964/j.issn.1000-0593(2020)12-3748-07

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