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Journal of Innovative Optical Health Sciences, Volume. 11, Issue 1, 1730002(2018)

Super-resolution fluorescence polarization microscopy

Karl Zhanghao1,2, Juntao Gao3,4, Dayong Jin5, Xuedian Zhang1,2、*, and and Peng Xi1,2,5
Author Affiliations
  • 1Department of Biomedical Engineering, College of Engineering Peking University, China
  • 2School of Optical-Electronic and Computer Engineering Shanghai University of Science and Technology, China
  • 3Department of Automation, Tsinghua University, Beijing 100084, China
  • 4Bioinfomatics Division, TNLIST MOE Key Laboratory of Bioinformatics and Center for Synthetic & System Biology, Tsinghua University, Beijing 100084, China
  • 5Faculty of Science, Institute for Biomedical Materials and Devices (IBMD) University of Technology, Australia
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    Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.

    Keywords
    fluorescence anisotropyFluorescence polarization microscopylinear dichroismpolarization modulationsuper resolution
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    Karl Zhanghao, Juntao Gao, Dayong Jin, Xuedian Zhang, and Peng Xi. Super-resolution fluorescence polarization microscopy[J]. Journal of Innovative Optical Health Sciences, 2018, 11(1): 1730002

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    Paper Information

    Received: Mar. 28, 2017

    Accepted: May. 8, 2017

    Published Online: Sep. 17, 2018

    The Author Email: Zhang Xuedian (zhangxuedian@hotmail.com)

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    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology