Chinese Optics Letters, Volume. 15, Issue 9, 090007(2017)

Feasibility study of phase-sensitive imaging based on multiple reference optical coherence tomography

Roshan Dsouza1,2, Hrebesh Subhash3, Kai Neuhaus1, Paul M. McNamara1,2, Josh Hogan4, Carol Wilson4, and Martin J. Leahy1,5、*
Author Affiliations
  • 1Tissue Optics and Microcirculation Imaging Group, School of Physics, National University of Ireland, Galway H91 CF50, Ireland
  • 2Compact Imaging Ireland Ltd., Galway H91 CF50, Ireland
  • 3Colgate-Palmolive Global Technology Centre, Piscataway NJ 08855, USA
  • 4Compact Imaging, Inc., Mountain View 94043, USA
  • 5Royal College of Surgeons (RCSI), Dublin, Ireland
  • show less
    Figures & Tables(8)
    Experimental setup of a phase-sensitive MR-OCT system based on a bulk optics configuration. L1–L4, lenses; BS, beam splitter; ND, neutral density filter; AMP, VCM amplifier; OW; optical window; GD, galvo driver; CT, capillary tube; SP, syringe pump.
    Flow chart for MR-OCT phase data processing. Signal processing includes a band-pass filter for each order of reflection m. The phase signal is derived from the complex signal by use of a Hilbert transformation.
    Structural and phase difference images of a 300 μm capillary tube filled with 5% intralipid solution with a Doppler angle of 50°. (a) The successive structural images corresponding to twelve orders of reflection. The scan range for the first order was ∼60 μm and the twelfth order was ∼720 μm. (b) Stitched image of all twelve orders shown in (a). (c) The corresponding phase difference images of all orders shown in (a) and (d) the stitched phase difference image. Scale bar: 250 μm.
    Measured system sensitivity of the MR-OCT system for twelve orders of reflection. The measurement was recorded with a mirror as a sample and by placing a neutral density filter (OD=2) in the sample arm.
    Determination of phase sensitivity calculating the standard deviation on the phase fluctuations. (a) The phase fluctuations between the sequential A lines of the first-order reflection. (b) The calculated phase sensitivity of the MR-OCT system for twelve orders of reflection. The phase difference was recorded using a static mirror as the sample and by placing a neutral density filter (OD=2) in the sample arm path.
    MR-OCT phase difference images of 5% intralipid solution flowing through a capillary tube measured at various flow rates with a Doppler angle of 50°. (a) Structural image of a 300 μm capillary tube filled with intralipid solution. (b) Phase difference image at 0 μm/s and (c)–(h) phase difference images at 50, 100, 150, 200, 250, and 300 μm/s. PW, phase wrap. Scale bar: 250 μm.
    Validation of the phase measurements that are shown in Fig. 6. (a) Phase profile along the center of the capillary tube (horizontal) and the corresponding second-order polynomial fit at each flow rate. (b) Comparison of measured and pump velocity.
    In vivo chicken embryo imaging with phase-sensitive MR-OCT system. (a) Structural image of a chicken embryo vessel and (b) the corresponding phase difference image. (c) The A line and phase profile taken at two different positions P1 and P2. Scale bar: 500 μm.
    Tools

    Get Citation

    Copy Citation Text

    Roshan Dsouza, Hrebesh Subhash, Kai Neuhaus, Paul M. McNamara, Josh Hogan, Carol Wilson, Martin J. Leahy. Feasibility study of phase-sensitive imaging based on multiple reference optical coherence tomography[J]. Chinese Optics Letters, 2017, 15(9): 090007

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Special Issue: ADVANCES IN METASURFACES

    Received: May. 13, 2017

    Accepted: Jul. 28, 2017

    Published Online: Jul. 19, 2018

    The Author Email: Martin J. Leahy (martin.leahy@nuigalway.ie)

    DOI:10.3788/COL201715.090007

    Topics