Photonics Research, Volume. 2, Issue 3, 87(2014)

Digital holographic microscopy with phase-shift-free structured illumination

Juanjuan Zheng1, Peng Gao1,2, Baoli Yao1、*, Tong Ye3, Ming Lei1, Junwei Min1, Dan Dan1, Yanlong Yang1, and and Shaohui Yan1
Author Affiliations
  • 1State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710119, China
  • 2Institut fur Technische Optik, Universitat Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany
  • 3Department of Bioengineering, Clemson University, Clemson-MUSC Bioengineering Program, Charleston, South Carolina 29425, USA
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    Figures & Tables(4)
    Schematic of DHM with structured illumination.
    Resolution enhancement of DHM by the structured illumination. (a) Spectrum evolvement of the object wave during the imaging process. (b) Flowchart of the iteration process for resolution enhancement. The black dots denote high frequency components of the object; the red-dashed circles denote the NA of the optical system.
    Simulation results for resolution enhancement by structured illuminations. (a) Amplitude and (b) phase distributions of the simulated specimen. (c) Intensity distribution of the structured illumination along horizontal orientation. (d) and (e) Reconstructed amplitude images by using on-axis plane wave illumination and by structured illuminations with the proposed iterative method, respectively.
    Experimental results for resolution enhancement by structured illuminations. (a) Four phase gratings with different orientations loaded on SLM to generate structured illuminations. (b) Recorded hologram under one structured illumination. (c) and (d) The reconstructed phase images by using the on-axis plane wave illumination and by using the proposed iteration method, respectively.
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    Juanjuan Zheng, Peng Gao, Baoli Yao, Tong Ye, Ming Lei, Junwei Min, Dan Dan, Yanlong Yang, and Shaohui Yan. Digital holographic microscopy with phase-shift-free structured illumination[J]. Photonics Research, 2014, 2(3): 87

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    Paper Information

    Category: Holography

    Received: Mar. 25, 2014

    Accepted: Apr. 24, 2014

    Published Online: Nov. 5, 2014

    The Author Email: Baoli Yao (yaobl@opt.ac.cn)

    DOI:10.1364/PRJ.2.000087

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