Research and Design of Bacterial Autofluorescence Detection Device

Jiayun ZHENG; Yikun WANG; Jingshu NI; Yang ZHANG; Yao HUANG; Yuanzhi ZHANG; Quanfu WANG; Xia WANG; Yong LUI; Jinhua ZHOU; Meili DONG

doi:10.3788/gzxb20235206.0617001

Acta Photonica Sinica, Volume. 52, Issue 6, 0617001(2023)

Research and Design of Bacterial Autofluorescence Detection Device

Jiayun ZHENG^1,2, Yikun WANG^2,3, Jingshu NI², Yang ZHANG², Yao HUANG^2,3, Yuanzhi ZHANG^2,3, Quanfu WANG², Xia WANG², Yong LUI^2,3、*, Jinhua ZHOU^1、**, and Meili DONG^2、***

Author Affiliations

¹School of Biomedical Engineering, Anhui Medical University, Hefei 230032, China

²Anhui Provincial Engineering Laboratory for Medical Optical Diagnosis & Treatment Technology and Instrument, Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China

³Wanjiang Center for Development of Emerging Industry Technology, Tongling 244000, China

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Figures & Tables(8)

Fig. 1. System diagram

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Fig. 2. Schematic of regular and irregular image processing

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Fig. 3. Three-dimensional fluorescence spectrum of common bacteria in wound

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Fig. 4. Imaging uniformity experiment

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Fig. 5. Fitting results of porphyrin gradient concentration

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Fig. 6. Fitting results of bacterial gradient concentration

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Fig. 7. Natural light and fluorescence image of wound model

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Table 1. Protoporphyrin IX dilution multiples
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Table 1. Protoporphyrin IX dilution multiples
Sample of proporphyrin IX Dilution multiples
1 10
2 50
3 100
4
5
300
500
6 600
7 800
8 1 000
9 1 200
10 1 500

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Jiayun ZHENG, Yikun WANG, Jingshu NI, Yang ZHANG, Yao HUANG, Yuanzhi ZHANG, Quanfu WANG, Xia WANG, Yong LUI, Jinhua ZHOU, Meili DONG. Research and Design of Bacterial Autofluorescence Detection Device[J]. Acta Photonica Sinica, 2023, 52(6): 0617001

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