The beam wander and spot radius of sodium laser beacon (SLB) have direct effects on correction of adaptive optics. Based on wander variance and effective radius model of SLB, the differences in these aspects between SLB and laser are investigated under the three atmospheric turbulence models by the numerical simulation method. Then, the average effective radius of SLB spot for the long-exposure and short-exposure is calculated for different laser launch diameters. Effects of laser with repumping power on beam wander and spot radius of SLB are analyzed. Results show that the strength of atmospheric turbulence, laser launch diameters and the repumping power have influence on beam wander and spot radius of SLB.

The refractive index structure parameter C2n from April to May in 2014 measured by the temperature pulsating instrument and sonic anemometers in Chengdu was statistically analyzed. The results of C2n measured by two methods agree well with each other both in day and at night. Compared with Hefei, the diurnal variation of the C2n is obvious but conversion time is different. Between 10-15 and 10-13 level, C2n frequencies measured by the temperature pulsating instrument and sonic anemometers are 74.05% and 84.40% respectively; the frequencies less than 10-15 are 24.07% and 10.63% respectively; the frequencies greater than 10-13 are 1.89% and 4.97% respectively. The relationship of C2n measured by sonic anemometers and temperature pulsating instrument is y=－8.5694×10－16+0.6520x, the correlation coefficient is 99.24%, and the possible causes of the C2n deviation measured by two methods was analyzed in brief.

Designing experiments to research joint toxicity of multi-heavy-metal to algal is beneficial to illuminate toxic process and mechanism of heavy metals stress algal in real environment. Chlorophyll fluorescence analysis technique and 24 factorial design method were used to design and analyze joint toxicity of heavy metal Hg2 + , Cd2 + , Cu2 + and Zn2 + to chlorella pyrenoidosa. Results show that the main effects of Hg, Cd, Cu, Zn and interaction effects of Hg* Cu, Hg* Zn and Hg* Cu* Zn influence chlorella pyrenoidosa photosynthetic fluorescence parameters Fv/Fm significantly (Sig.<0.05). The relationship between the concentration of heavy metals and photosynthesis inhibition rate was established by using multiple linear regression method. The model is as follows: y［%］=－22.557+19.926Hg+5.282Cu+0.015Zn+0.029Hg*Zn－ 0.002Hg*Cu*Zn, and it can be used to account for mechanism of heavy metals stressed chlorella pyrenoidosa. To test and verify the model by statistical method and experiment, the results show that the model can be used for estimating the joint toxicity of Hg2 + , Cd2 + , Cu2 + and Zn2 + stress chlorella pyrenoidosa in laboratory conditions.

It is difficult to extract each component from overlapping three-dimensional fluorescence spectra of mixture. Considering intrinsic nonnegativity constraints on spectra, three-dimensional fluorescence spectra data of polycyclic aromatic hydrocarbons (PAHs) mixtures of phenanthrene, pyrene and anthracene is analyzed by using projected gradient and alternating least square algorithms based on nonnegative matrix factorization (NMF) by taking the results of K-means clusting as initial values. The negative data of separated spectra is eradicated. Three-dimensional fluorescence spectra of each component is extracted, and the similarity coefficients between computed spectra and its corresponding standard spectra are computed, which is greater than 0.970. Results demonstrate that three components are recognized accurately by NMF, which could overcome the interference caused by overlapping spectra and extract spectral components effectively. Alternating least square algorithms based on NMF is more suitable for online real-time monitoring.

Scattering coefficients of atmospheric aerosol is not only an indicator of atmospheric pollution, but also one of the important parameters of climate change. A method of aerosol scattering coefficient measurement based on integral turbidity as well as system design was proposed. The system design principle, hardware and software design and other aspects of the system design and implementation process were introduced respectively. To ensure the stability of the light source, constant current drive mode was used. Light scattering signals was detected by high speed single photon counting technology. Finally, linearity and stability of light source and accuracy of the detector were verified through experiment. Feasibility and reliability of the system were proved by comparing with a forward-scattering visibility sensor HW-N1.

In order to facilitate the subsequent research of remote sensing images, a high-resolution remote sensing image restoration algorithm based on wavelet transform and iterative shrinkage (IST) has been implemented. Considering that the restoration process in the algorithm needs large memory, memory-mapped file method is used to map the high-resolution remote sensing image into the process address space. Because of the edge jump phenomenon which affects the image quality after splicing image block, a special splicing strategy is used for block restoration. Restoration algorithm is implemented in the VC platform. Through remote sensing image restoration experiments, analysis and evaluation of image restoration, the restoration algorithm is proved to have good recovery performance and efficiency.

Based on the vector radiative transfer model-VLIDORT, the polarization characteristics of haze by using haze optical properties under different conditions are calculated. On the analysis and comparison of polarization characteristics under the common aerosol models and haze aerosol, the sensitive of geometry, aerosol optical thickness, aerosol complex refractive index, single scattering albedo and surface parameters are obtained. Theoretical references for haze aerosol monitoring, retrieving aerosol optical depth and aerosol complex refractive index by using polarized radiation are provided.

In the analysis of high resolution melting (HRM) of microfluidic fluorescence PCR amplification products, fluorescence intensity has a sudden change with the delink of DNA chain, while whether the abrupt change can be detected is dependent on the accuracy of temperature control. A kind of multi-threshold PID algorithm was put forward, and a temperature control system based on FPGA was designed to adapt to the analysis of high resolution melting. Based on the difference of the measurement value and the set value, the temperature change can be controlled accurately through changing the pulse-time ratio of pulse width modulation (PWM) wave and the control frequency of the algorithm from the FPGA circuit system. After temperature calibrations of the measurement system, the accuracy precedes 0.1℃ and the resolution achieves ±0.01℃. The result of HRM experiment shows that this system can detect the single base mutation of DNA sequence successfully, and the temperature control system can meet the design requirements.

Cavity ring-down spectroscopy (CRDS) was used widely in trace gas detection and mirror reflectivity measurement due to the characteristics of high sensitivity, high precision and simple device. A CRDS set was established based on the continuous wave. Pound-Drever-Hall (PDH) frequency laser locking technique was utilized to lock the frequency of the laser on the eigen frequency of the cavity, so as to achieve the goal of wavelength stability of laser. By observing the transmission signal with different frequency of switch signal bearing on the AOM, optimal cut-off frequency 60 kHz was gotten. Then the system was used to measure the reflectivity of the cavity mirrors. Compared with the measurement results without locking, the results indicats that the locking case has less drift and the error is compressed into one third.

A method is presented for atmospheric CO2 balloonsonde measurement. Based on Beer-Lambert law and non-dispersed infrared(NDIR) technique, an experimental device is designed and developed. An infrared LED is used to produce suitable light source, and infrared LED detectors and a proper designed electronic circuit are used to transform light intensity into electrical signals in the experimental device. Calibration method for CO2 measurement is also presented. A comparison experiment is carried out for continuous 12 days on ground with EC9820 analyzer. Result shows that the trend of the measurement data of 12 days is consistent, and standard deviation is 9.835 ppm, which basically satisfies the precision demand for atmospheric CO2 measurement. The feasibility of CO2 measurement has been validated.