In recent years, microbial induced calcium carbonate precipitation (MICP) has become an efficient and environmentally friendly technology for repairing concrete cracks. In this paper, a mineralization microorganism that grows in a low temperature environment was selected, and then the changes of Ca2+ concentration, pH value and calcification rate were studied in alkaline solution to reveal the dynamic process of microbial induced mineralization. Subsequently, the microorganism was applied to the self-healing of concrete cracks, and the crack healing ratio and permeability coefficient were used to characterize the self-healing effect of concrete cracks. The results show that the microorganism quickly reduces the Ca2+ concentration in the solution and increases the calcification rate. Meanwhile, the mineralization products are mainly spherical calcite CaCO3. For the early concrete cracks with the width of less than 0.50 mm, the surface of the crack basically fills with white precipitations after 28 d healing time at the curing temperature of 10 ℃, and the permeability coefficient drops by two orders of magnitude. In addition, the healing products at the crack mouth are mainly spherical calcite CaCO3 with different sizes.