There is abundant material information in biological samples, and each material has its unique properties and functions. Research on the production, transportation, and distribution of these materials in organisms can give us a deep understanding of biological systems. Optical microscopes with chemical specificity are powerful and reliable tools for these studies. Raman scattering technology has greatly promoted the development of biomedicine because it solves the problems caused by the fluorescence microscope. Especially, hyperspectral coherent Raman scattering (HS-CRS) microscope can perform label-free chemical imaging of substances with different components. By providing image and spectral information at the same time, the properties, content and distribution of substances in the sample can be determined. It provides scientific researchers with more dimensional material information. In recent decades, HS-CRS has been continuously updated and applied in the biomedical field. This paper introduces the main HS-CRS implementation and schemes and summarizes its application in the biomedical field.