Laser speckle flowgraphy (LSFG) technique, also known as laser speckle contrast analysis (LSCA), is a full field optical imaging method without scanning, which can quantitatively analyze the change of blood flow. LSCA method is commonly used in space, time and the ratio of contrast lining spatial-temporal speckle contrast analysis method. We analyze the applications and the accuracy of the previous researches, in which the analysis methods lack of three different contrast factors. Through the use of TiO2 containing suspended particles in the fluid through a hose simulated biological changes in the blood flow, we introduce the principle of LSFG technique, and compare the methods of space, time and spatial and temporal contrast analysis. We implement the various different speeds to compare the results of various contrast analysis methods. The experimental results show that the spatial-temporal contrast analysis method compared to the simple spatial contrast or the simple time contrast test range can guarantee the time resolution and high spatial resolution at the same time, reduce the computation of data processing, and improve the accuracy of the speckle contrast.