Optical Instruments, Volume. 37, Issue 6, 550(2015)
Imaging technology in modern microsystems
The imaging technology in modern microscope for widefield microscope, confocal microscope and super-resolution microscope is outlined here. The most frequently-used technology in the widefield microscope is brightfield, darkfield, phase contrast, polarization, DIC, modulation contrast and fluorescence. In phase contrast, there is also apodized phase contrast. In addition, DIC, C-DIC and PlasDIC are also presented. Confocal microscope is developed greatly with the development of computer and manufacture technology. Besides conventional confocal system, the principle of coherent anti-Stokes Raman scattering(CARS) confocal,multi-photo confocal, white confocal are also explained in detail. As for super-resolution microscope, the principle of stimulated emission depletion(STED) and ground state depletion microscopy followed by individual molecule return(GSDIM) are clarified.
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ZHANG Xiangxiang. Imaging technology in modern microsystems[J]. Optical Instruments, 2015, 37(6): 550
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Received: Mar. 12, 2015
Accepted: --
Published Online: Jan. 19, 2016
The Author Email: Xiangxiang ZHANG (carol.zhang@leica-microsystems.com)