This paper reports the observation of the blue-green up-conversion luminescence phenomenon of HoP5O14 noncrystalline induced by pulse DCM dye laser. From the experimental measurement, theoretical analysis and calculation on the up-conversion phenomenon, mechanism and passenges, it has shown that the up- conversion luminescence of 5F3 and 5S2 level is resulted mainly from energy transfer between Ho3+ ions, and the up-conversion luminescence of 5G4and (5G3G)5level is caused by both of the sequential two- photon absorption of single H3+0 ion and energy transfer between H3+0 ions.