This study investigated the manufacturing of micro-holes in Inconel 718 material using high-temperature chemical-assisted nanosecond laser machining. The study examined the effects of various pulse numbers on the diameter, depth, and wall characteristics of micro-holes in both ambient air and high-temperature chemical environments. Additionally, the study compared the morphological differences of micro-holes under ambient air and high-temperature chemical conditions and thoroughly investigated the material removal process involved in fabricating micro-holes using high-temperature chemical-assisted nanosecond laser machining. The experimental results demonstrated that using lower pulse frequencies had a beneficial impact on enhancing the morphology of micro-holes in high-temperature chemical environments. Finally, the study investigated the influence of pulse delay on the laser machining of micro-holes with high-temperature chemical etching assistance and analyzed the patterns of pulse delay in micro-hole formation. The results show that when the delay time is 1 s, the total processing time is 15 s, the roundness of the hole improves to 0.981, the hole wall becomes smoother, and the heat-affected zone decreases accordingly. Increasing a certain pulse delay can achieve high -quality micropores without recast layer, with a smaller heat-affected zone and better roundness.