Laser & Optoelectronics Progress, Volume. 52, Issue 1, 10003(2015)

Structured Illumination Fluorescence Microscopy: Diffraction-Limit Breaking Principle and Application in Life Science

Wu Meirui1,2,3、*, Yang Xibin1,2,3, Xiong Daxi1, Li Hui1, and Wu Xiaodong1
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
  • 3[in Chinese]
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    Resolution of traditional fluorescence microscopy is limited by the diffraction of light. Diffraction limit can be broken by structured illumination to get higher resolution. Compared to other super-resolution microscopy techniques, structured illumination fluorescence microscopy can achieve higher imaging speed and need a simple setup, which has an important application life science research. In this paper, we first illustrate the principle and reconstruction algorithm to obtain 2D and 3D super-resolution images as well as non-linear structured illumination. Then the generally used structured illumination method and setup based on grating, spatial light modulator (SLM) as well as digital micro-mirror device (DMD) are introduced and compared. At last we summarize the application of structured illumination fluorescence microscopy for observing biological structures and processes.

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    Wu Meirui, Yang Xibin, Xiong Daxi, Li Hui, Wu Xiaodong. Structured Illumination Fluorescence Microscopy: Diffraction-Limit Breaking Principle and Application in Life Science[J]. Laser & Optoelectronics Progress, 2015, 52(1): 10003

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    Paper Information

    Category: Reviews

    Received: May. 22, 2014

    Accepted: --

    Published Online: Dec. 30, 2014

    The Author Email: Meirui Wu (meirui_wu@163.com)

    DOI:10.3788/lop52.010003

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