Er³⁺/Ce³⁺co-doped tellurite glasses with composition of TeO₂-GeO₂-Li₂O-Nb₂O₅were prepared using conventional melt-quenching technique for potential applications in Er³⁺-doped fiber amplifier (EDFA). The absorption spectra, up-conversion spectra and 1.53 μm band fluorescence spectra of glass samples were measured. It is shown that the 1.53 μm band fluorescence emission intensity of Er³⁺-doped tellurite glass fiber is improved obviously with the introduction of an appropriate amount of Ce³⁺, which is attributed to the energy transfer (ET) from Er³⁺to Ce³⁺. Meanwhile, the 1.53 μm band optical signal amplification is simulated based on the rate and power propagation equations, and an increment in signal gain of about 2.4 dB at 1 532 nm in the Er³⁺/Ce³⁺co-doped tellurite glass fiber is found. The maximum signal gain reaches 29.3 dB on a 50 cm-long fiber pumped at 980 nm with power of 100 mW. The results indicate that the prepared Er³⁺/Ce³⁺co-doped tellurite glass is a good gain medium applied for 1.53 μm broadband and high-gain EDFA.