Acta Optica Sinica, Volume. 27, Issue 6, 1076(2007)
Computation for Optical Trap Stiffness Versus Refractive Index of Biological Cell
In order to study the methods for measuring the refractive index of a biological cell based on optical tweezers technology, a ray-optics model has been used to predict the relationship between the trap stiffness and the refractive index of an ideal cell as a spherically symmetric Rayleigh sphere (radius a=10 μm, refractive indices n=1.35~1.70) in a single-beam gradient optical trapping (laser wavelength λ=780 nm, power P= 6 mW, beam waist radii ω(0)=0.6 μm, 0.8 μm and 1.0 μm). Numerical simulation shows that the envelope of each curve is a cubic polynomial fitted well with the cell refractive indices. Results indicate that the calibration process for parameters of a cubic polynomial must be performed on four standard particles before the cell refractive index is obtained by measuring its trap stiffness.
Get Citation
Copy Citation Text
[in Chinese], [in Chinese], [in Chinese]. Computation for Optical Trap Stiffness Versus Refractive Index of Biological Cell[J]. Acta Optica Sinica, 2007, 27(6): 1076