In order to improve the detection sensitivity of glucose sensor in human environment (pH=7.4), a long period grating glucose sensor based on phenylboronic acid derivative is proposed. 4-vinylphenylboronic acid is polymerized on the surface of long period grating by C=C double bond recombination to measure glucose concentration. The experimental results show that the central wavelength of the glucose sensor can be reset to the original point after multiple repeated use, the sensitivity of the glucose sensor is 0.34 nm/(mg?mL-1) in the range of pH=7.4 and concentration of 0.1~3 mg/mL. Besides, the results show that the glucose sensor has good consistency and stability in repeated and long-term glucose determination.