Real-time monitoring the gelatinization process of single rice starch particle using laser tweezers Raman spectroscopy system under the condition of excessive water and special heating mode is reported. These Raman spectra of gelatinization process are acquired using an imaging CCD spectrograph and the gelatinization process is remarked by the changes of spectra peak height. The experimental result further confirmed the fact that the band of 477cm－1 is assigned as skeletal mode. The rate of gelatinization about single rice starch granules is studied by analyzing the C-O-H group related characteristic peaks which locate at the band of 1 052, 1 083, 1 127, 1 339 cm－1. The results demonstrate that the rate of gelatinization accelerates with rising temperature until the end of gelatinization process.