In order to solve the problem that large deviation of the refraction index and temperature coefficient of refraction index obtained by regression calculation from the experimentally measured ones comes out when ZEMAX method is used for solving these empirical formula constants and regression calculation, 1stOpt differential evolution was used to calculate the temperature coefficient of refraction index empirical formula constants. With fluoro-crown D-FK61 and flint H-TF3A optical glass as examples, the λtk coefficients calculated by the proposed method are consistent with the general numerical range of 0.08~0.33, and the difference of refractive index and its temperature coefficient between the value obtained by regression calculation method and the measured value is less than 1×10－5 and 2×10－7/℃, respectively. As an effective supplement of ZEMAX software to calculate the temperature coefficient of refractive index of the optical glasses, the proposed method greatly improves the accuracy and can provide accurate optical parameter guarantee for thermal compensation optical system design.