Under the same driving voltage,the colorful liquid crystal display device (CLCD) has quite different electro-optical characteristics at different wavelength. As a result,the saturation of colorful display image could be greatly influenced. The electro-optical characteristics of negative TN-LCD,STN-LCD and VA-LCD driven by different voltage are measured by UV-Vis8500 double beam ultraviolet (UV)/visible spectrophotometer,from which the electro-optical and threshold characteristics of three primary colors and the gradient variation with wavelength are analyzed and compared. The results show that with the increasing of wavelength,Vth,Vsat and V50 of TN-LCD decrease. And the decreasing speed of them is basically same. With the increasing of wavelength,Vth,Vsat and V50 of STN-LCD decrease. And the decreasing speed of them is different in each wavelength. With the increasing of wavelength,Vth,Vsat and V50 of VA-LCD increase gradually. But the increasing speed of Vsat is faster than that of V50 and the increasing speed of V50 is faster than that of Vth. The chosen driving voltages of three commonly used different negative liquid crystal display devices are compared and analyzed. The results can provide certain guidance for choosing the driving voltage of colorful liquid crystal display (CLCD).