A simplified ring cavity for achieving a unidirectional room temperature multi-wavelength erbium-doped fiber ring laser without optical isolator is demonstrated. The fiber ring cavity is built in such a way that the optical fields propagating in two directions suffer different losses caused by one sampled fiber Bragg grating. Furthermore, simultaneous multi-wavelength lasing with 0.8-nm intervals is demonstrated with sinusoidal phase modulation just before the sampled fiber Bragg grating to prevent single-wavelength lasing and unstable wavelength oscillation.