Laser & Optoelectronics Progress, Volume. 59, Issue 24, 2418001(2022)
Focusing Spectral CARS Microscopic Imaging in Low Fingerprint Regions
A spectral focusing coherent anti-Stokes Raman scattering (CARS) microimaging system was developed using a femtosecond oscillator as the excitation source. Pump light and Stokes light were obtained by using dichromatic mirrors. Two- and three-dimensional microscopic imaging studies of the spectral focusing CARS imaging in the low-fingerprint region of polystyrene samples were carried out. While changing the delay time of pump-Stokes during two-dimensional imaging, CARS signal intensity spectra that varied with delay time were obtained. The switching of excited Raman vibrations in the process of changing the pump-Stokes delay time was analyzed by comparing it to the polystyrene Raman spectra. Furthermore, the three-dimensional CARS imaging was realized by tomography in the fixed Raman vibration mode for polystyrene samples.
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Ning Zhang, Guozhong Hou, Yanyan Deng, Shuo Liu, Yulei Wang, Lü Zhiwei, Yuanqin Xia. Focusing Spectral CARS Microscopic Imaging in Low Fingerprint Regions[J]. Laser & Optoelectronics Progress, 2022, 59(24): 2418001
Category: Microscopy
Received: Apr. 8, 2022
Accepted: Apr. 27, 2022
Published Online: Nov. 30, 2022
The Author Email: Xia Yuanqin (xiayq@hebut.edu.cn)